Department of Biomedical Engineering, Division of Endocrinology, Diabetes and Metabolism, Diabetes Research Institute, Leonard M. Miller School of Medicine, University of Miami, 1450 NW 10th Ave, Miami, Florida 33136, United States.
J Phys Chem B. 2011 Sep 8;115(35):10547-52. doi: 10.1021/jp204146n. Epub 2011 Aug 15.
Perfluorocarbons (PFCs) are compounds with increased oxygen solubility and effective diffusivity, making them ideal candidates for improving oxygen mass transfer in numerous biological applications. Historically, quantification of the mass transfer characteristics of these liquids has relied on the use of elaborate laboratory equipment and complicated methodologies, such as in-line gas chromatography coupled with temperature-controlled glass fritted diffusion cells. In this work, we present an alternative method for the determination of dissolved oxygen content in PFC emulsions and, by extrapolation, pure PFCs. We implemented a simple stirred oxygen consumption microchamber coupled with an enzymatic reaction for the quantitative determination of oxygen by optical density measurements. Chambers were also custom fitted with lifetime oxygen sensors to permit simultaneous measurement of internal chamber oxygen levels. Analyzing the consumption of oxygen during the enzymatic reaction via recorded oxygen depletion traces, we found a strong degree of correlation between the zero-order reaction rate and the total measured oxygen concentrations, relative to control solutions. The values obtained were in close agreement with published values in the literature, establishing the accuracy of this method. Overall, this method allows for easy, reliable, and reproducible measurements of oxygen content in aqueous solutions, including, but not limited to PFC emulsions.
全氟碳化合物（PFCs）是一类具有高氧气溶解度和有效扩散系数的化合物，这使它们成为提高许多生物应用中氧气传质的理想候选物。历史上，这些液体的传质特性的量化一直依赖于使用复杂的实验室设备和复杂的方法，例如在线气相色谱法与控温玻璃烧结扩散池结合使用。在这项工作中，我们提出了一种替代方法，用于测定 PFC 乳液和纯 PFC 中的溶解氧含量。我们实施了一种简单的搅拌氧消耗微腔，并结合酶反应，通过光密度测量进行定量氧测定。腔室还经过定制，配备了寿命氧传感器，以允许同时测量内部腔室氧水平。通过记录的氧气消耗痕迹分析酶反应期间的氧气消耗，我们发现零级反应速率与相对于对照溶液测量的总氧气浓度之间存在很强的相关性。所得值与文献中公布的值非常吻合，从而验证了该方法的准确性。总体而言，该方法可轻松、可靠且可重复地测量水溶液中的氧气含量，包括但不限于 PFC 乳液。
