Lupo Anthony, Mozola Mark
Neogen Corp., 620 Lesher Pl, Lansing, MI 48912, USA.
J AOAC Int. 2011 May-Jun;94(3):886-99.
Neogen Corp. has developed an improved Veratox histamine test kit for the detection of histamine in tuna tissue. The purpose of this study was to validate the method under the requirements of AOAC Research Institute (RI) Performance Tested Methods. Three AOAC Official Methods for histamine (954.04, 957.07, and 977.13) and one ELISA method have been performance tested by the AOAC RI. The most popular is AOAC Official Method 977.13, the fluorometric method, which is considered the reference method, but is complicated and time-consuming. The need for a rapid ELISA test kit to be validated by the AOAC RI exists.
Neogen公司开发了一种改良的Veratox组胺检测试剂盒，用于检测金枪鱼组织中的组胺。本研究的目的是根据AOAC研究机构（RI）性能验证方法的要求对该方法进行验证。AOAC RI已对三种AOAC组胺官方方法（954.04、957.07和977.13）和一种ELISA方法进行了性能验证。最常用的是AOAC官方方法977.13，即荧光法，它被视为参考方法，但操作复杂且耗时。因此需要一种快速ELISA检测试剂盒并由AOAC RI进行验证。
