Lupo Anthony, Roebuck Chris, Settimo Ken, Quain Anna, Kennedy Justina, Abouzied Mohamed
Neogen Corp., 620 Lesher Pl, Lansing, MI 48912, USA.
J AOAC Int. 2010 Mar-Apr;93(2):600-10.
Neogen Corp. developed the Veratox DON test kit for the detection ofdeoxynivalenol (DON). The purpose of this study was to validate the method under the requirements of the AOAC Research Institute Performance Tested Methods (PTM) program. There are two AOAC Official Methods for DON detection: 986.17 and 986.18, the first of which is a TLC method and the second a GC method. A rapid method (PTM 000701) has also been performance tested by the AOAC Research Institute. One of the most widely used reference methods; however, is a GC method with electron capture detection that is referred to as the reference method in this paper. Although considered the reference method, the GC procedure is complicated and requires the investment of both expensive equipment and a highly skilled technician. A rapid (e.g., ELISA) test kit needs to be validated by the AOAC Research Institute. The Veratox 2/3 method is highly reproducible with average CV values < 10%, and is very accurate, showing > 97% correlation to reference methods.
Neogen公司开发了用于检测脱氧雪腐镰刀菌烯醇（DON）的Veratox DON检测试剂盒。本研究的目的是根据AOAC研究机构性能验证方法（PTM）计划的要求验证该方法。有两种AOAC官方DON检测方法：986.17和986.18，第一种是薄层色谱法，第二种是气相色谱法。AOAC研究机构还对一种快速方法（PTM 000701）进行了性能验证。然而，最广泛使用的参考方法之一是一种带有电子捕获检测的气相色谱法，本文将其称为参考方法。尽管被视为参考方法，但气相色谱程序复杂，需要投入昂贵的设备和高技能的技术人员。快速（如酶联免疫吸附测定）检测试剂盒需要由AOAC研究机构进行验证。Veratox 2/3方法具有高度可重复性，平均变异系数值<10%，并且非常准确，与参考方法的相关性>97%。
