Institut National de la Santé et de la Recherche Médicale, U, Cordeliers Research Center, Paris, France.
Arterioscler Thromb Vasc Biol. 2011 Oct;31(10):2322-30. doi: 10.1161/ATVBAHA.111.230979. Epub 2011 Jul 28.
Studies suggest the implication of CD16(+) subpopulations (CD14(+)CD16(+), CD14(dim)CD16(+)) in inflammatory diseases. We aimed to determine the frequency of these subpopulations during weight loss in obesity and diabetes, conditions associated with changes in systemic inflammation, and we tested the link with subclinical atherosclerosis.
CD14(dim)CD16(+) and CD14(+)CD16(+) frequencies were measured by flow cytometry in lean subjects, obese subjects before and after a hypocaloric diet or gastric surgery, and obese diabetic subjects before and after gastric surgery. Both monocyte subsets were increased in obese subjects, with a significant enrichment of the CD14(dim)CD16(+) subpopulation in obese diabetic patients. Multivariate analysis demonstrated a link between the percentages of CD14(dim)CD16(+) monocytes and glycemia, independent of fat mass. Drastic weight loss led to a sharp decrease of this subset, the variations of which were strongly related to fat mass changes. A reduction of at least 5% of fat mass was sufficient to observe a significant decrease of CD14(dim)CD16(+) monocytes. A diminution of the CD14(+)CD16(+) subset was also observed during weight loss and was associated with a decrease in intima-media thickness.
This work demonstrates a major impact of fat mass variations on CD14(dim)CD16(+) monocyte subsets and that the decrease in the CD14(+)CD16(+) subpopulation is linked to a reduction of subclinical atherosclerosis.
URL: http://clinicaltrials.gov. Unique identifier: NCT00476658.
研究表明 CD16(+)亚群（CD14(+)CD16(+)、CD14(dim)CD16(+)）在炎症性疾病中的作用。我们旨在确定这些亚群在肥胖和糖尿病减肥期间的频率，这些情况与系统性炎症变化有关，并检验它们与亚临床动脉粥样硬化的关系。
通过流式细胞术测量瘦受试者、低热量饮食或胃手术后肥胖受试者以及胃手术后肥胖糖尿病受试者中 CD14(dim)CD16(+)和 CD14(+)CD16(+)的频率。两种单核细胞亚群在肥胖者中均增加，肥胖糖尿病患者中 CD14(dim)CD16(+)亚群明显富集。多变量分析表明，CD14(dim)CD16(+)单核细胞的百分比与血糖之间存在联系，与脂肪量无关。体重急剧下降导致该亚群急剧减少，其变化与脂肪量变化密切相关。脂肪量减少至少 5%足以观察到 CD14(dim)CD16(+)单核细胞的显著减少。体重减轻期间也观察到 CD14(+)CD16(+)亚群减少，与内中膜厚度减少相关。
这项工作表明脂肪量变化对 CD14(dim)CD16(+)单核细胞亚群有重大影响，CD14(+)CD16(+)亚群的减少与亚临床动脉粥样硬化的减少有关。
