S-胸腺嘧啶-L-半胱氨酸的傅里叶变换红外光谱、傅里叶变换拉曼光谱及表面增强拉曼光谱研究

[FTIR, FT-Raman and surface enhanced Raman study of S-thyminyl-L-cysteine].

作者信息

Cai Peng-ying, Zhou Guang-ming, Yang Da-cheng

机构信息

Key Laboratory on Luminescence and Real-Time Analysis of the Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2011 May;31(5):1274-8.

PMID:21800581

Abstract

The present paper reports the FT-infrared spectra and FT-Raman spectra of the S-thyminyl-L-cysteine. The optimal concentration of the S-thyminyl-L-cysteine on silver sol is 10(-4) mol L(-1). The interaction of S-thyminyl-L-cysteine with Ag nanoparticles was adsorbed by COO-, NH3+, S, and ring in the tilted way. Amino peak was enhanced in the acidic condition, carboxyl peak was enhanced in alkaline condition. Adsorption of S-thyminyl-L-cysteine on silver nanoparticles was mainly with carboxyl under the acidic condition and with amino under the alkaline condition. Other groups had no change at different pH. Establishment of this adsorption model provided important information and useful reference for further Raman spectra study of PNA, peptides and other biological molecules.

摘要

本文报道了S-胸腺嘧啶-L-半胱氨酸的傅里叶变换红外光谱和傅里叶变换拉曼光谱。S-胸腺嘧啶-L-半胱氨酸在银溶胶上的最佳浓度为10(-4) mol L(-1)。S-胸腺嘧啶-L-半胱氨酸与银纳米颗粒的相互作用通过COO-、NH3+、S和环以倾斜方式吸附。在酸性条件下氨基峰增强，在碱性条件下羧基峰增强。在酸性条件下，S-胸腺嘧啶-L-半胱氨酸在银纳米颗粒上的吸附主要通过羧基，在碱性条件下主要通过氨基。其他基团在不同pH值下没有变化。该吸附模型的建立为进一步研究肽核酸、肽和其他生物分子的拉曼光谱提供了重要信息和有用参考。