使用伏安生物传感器诊断幽门螺杆菌细菌感染。

Diagnosis of Helicobacter pylori bacterial infections using a voltammetric biosensor.

机构信息

Biosensor Research Institute, Seoul National University of Science and Technology, Seoul, South Korea.

出版信息

J Microbiol Methods. 2011 Oct;87(1):44-8. doi: 10.1016/j.mimet.2011.07.002. Epub 2011 Jul 23.

DOI:10.1016/j.mimet.2011.07.002

Abstract

The voltammetric assay of Helicobacter pylori DNA was investigated using a bismuth-immobilized carbon nanotube electrode (BCNE). The analytical cyclic voltammetry (CV) peak potential was obtained at a 0.4V reduction scan, where the diagnostic optimum square-wave (SW) stripping working range was achieved at 0.72-7.92 μg/mL H. pylori DNA (11 points). A relative standard deviation of 1.68% (RSD, n=5) was obtained with 3.2 mg/mL H. pylori DNA using a 240 s accumulation time. Under optimum conditions, detection limit was 0.06 μg/mL. The developed sensors can be used for clinical application in the 15th doubted human gastric tissues, since the patient's peak current increased a hundred times more than the negative healthy tissue did. The sensing time obtained was only two minutes, and the process was simpler compared to common PCR amplification and electrophoresis photometric detection systems.

摘要

采用铋固定化碳纳米管电极（BCNE）对幽门螺杆菌 DNA 的伏安法测定进行了研究。在 0.4V 还原扫描时获得分析循环伏安（CV）峰电位，在该电位下，诊断最佳方波（SW）剥离工作范围在 0.72-7.92μg/mL H. pylori DNA（11 个点）下实现。使用 240s 的累积时间，3.2mg/mL H. pylori DNA 的相对标准偏差为 1.68%（RSD，n=5）。在最佳条件下，检测限为 0.06μg/mL。由于患者的峰电流比阴性健康组织增加了一百倍，因此开发的传感器可用于 15 个可疑人类胃组织的临床应用。与普通的 PCR 扩增和电泳光度检测系统相比，获得的检测时间仅为两分钟，且过程更简单。