用于鉴定李斯特菌属的Mast ID和API 50CH系统评估
Evaluation of the Mast ID and API 50CH systems for identification of Listeria spp.
作者信息
Kerr K G, Rotowa N A, Hawkey P M, Lacey R W
机构信息
Department of Microbiology, University of Leeds, West Yorkshire, United Kingdom.
出版信息
Appl Environ Microbiol. 1990 Mar;56(3):657-60. doi: 10.1128/aem.56.3.657-660.1990.
Abstract
A multipoint inoculation technique (Mast ID) for the identification and species determination of Listeria monocytogenes (sensu strictu) and six other species of the genus Listeria was evaluated. This was compared with the commercially available API 50CH system. Both methods successfully identified all 123 strains tested. The Mast ID system is inexpensive and utilizing a multipoint inoculation technique permits the screening of up to 21 isolates per 9-cm petri dish. The API 50CH system was more expensive and time consuming and is therefore suitable only for the examination of smaller numbers of strains.
摘要
评估了一种用于鉴定单核细胞增生李斯特菌（狭义）和李斯特菌属其他六个物种并确定其种类的多点接种技术（Mast ID）。将其与市售的API 50CH系统进行比较。两种方法均成功鉴定了所有123株测试菌株。Mast ID系统价格低廉，采用多点接种技术，每个9厘米培养皿最多可筛选21个分离株。API 50CH系统价格更高且耗时更长，因此仅适用于检测数量较少的菌株。
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