API李斯特菌鉴定系统，一种新型且有前景的用于鉴定李斯特菌分离株的一日系统。
API Listeria, a new and promising one-day system to identify Listeria isolates.
作者信息
Bille J, Catimel B, Bannerman E, Jacquet C, Yersin M N, Caniaux I, Monget D, Rocourt J
机构信息
Centre National de Référence des Listeria, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
出版信息
Appl Environ Microbiol. 1992 Jun;58(6):1857-60. doi: 10.1128/aem.58.6.1857-1860.1992.
Abstract
API Listeria is a new 10-test strip for 24-h biochemical identification of Listeria isolates. With this commercial system, 85% of 646 Listeria strains, including atypical isolates selected for this study, were recognized at the species and subspecies level without a complementary test. A new test differentiates Listeria monocytogenes from L. innocua on the basis of the absence of arylamidase from the former. With this system, 97.7% (252 of 258) of the L. monocytogenes strains tested were correctly identified and differentiated from 99.4% (175 of 176) of the L. innocua strains also tested. Gram-positive bacteria other than Listeria spp. gave quite different biochemical patterns. This system considerably reduced the time needed for conventional identification, since results were available within 18 to 24 h.
摘要
API李斯特菌检测条是一种新型的10项检测条，用于对李斯特菌分离株进行24小时生化鉴定。使用这个商业系统，在646株李斯特菌中，包括为本研究挑选的非典型分离株，85%在不进行补充检测的情况下就能在种和亚种水平上得到识别。一项新的检测基于前者缺乏芳基酰胺酶，将产单核细胞李斯特菌与无害李斯特菌区分开来。使用该系统，所检测的97.7%（258株中的252株）产单核细胞李斯特菌菌株被正确鉴定，并与同样检测的99.4%（176株中的175株）无害李斯特菌菌株区分开来。除李斯特菌属以外的革兰氏阳性菌呈现出截然不同的生化模式。该系统大大缩短了传统鉴定所需的时间，因为在18至24小时内就能得到结果。
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