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肉毒杆菌神经毒素A、B、E、F和G产生梭菌的PCR和基因探针鉴定及食品样本评估。

PCR and gene probe identification of botulinum neurotoxin A-, B-, E-, F-, and G-producing Clostridium spp. and evaluation in food samples.

作者信息

Fach P, Gibert M, Griffais R, Guillou J P, Popoff M R

机构信息

Centre National d'Etudes Vétérinaires et Alimentaires, Laboratoire Central d'Hygiène Alimentaire, Paris, France.

出版信息

Appl Environ Microbiol. 1995 Jan;61(1):389-92. doi: 10.1128/aem.61.1.389-392.1995.

DOI:10.1128/aem.61.1.389-392.1995
PMID:7887623
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC167297/
Abstract

A degenerate primer pair was selected to amplify specifically a 260-bp DNA fragment from Clostridium botulinum types A, B, E, F, and G, and five individual probes allowed identification of each toxinotype by hybridization of the PCR products. The 72 strains of different Clostridium species tested and 11 other bacterial species commonly found in food samples gave an amplification product. This assay was able to detect 1 C. botulinum type A or B and 10 C. botulinum type E strains per reaction. With 184 artificially contaminated food samples, after an 18-h enrichment step, the sensitivity was 10 bacteria per g of sample and the correlation with the mouse bioassay reached 95.6%.

摘要

选择一对简并引物，特异性扩增来自A型、B型、E型、F型和G型肉毒梭菌的260bp DNA片段，5种单独的探针通过PCR产物杂交可鉴定每种毒素型。所检测的72株不同梭菌属菌株以及食品样本中常见的其他11种细菌均产生了扩增产物。该检测方法每个反应能够检测到1株A型或B型肉毒梭菌以及10株E型肉毒梭菌。对于184份人工污染的食品样本，经过18小时的增菌步骤后，灵敏度为每克样本10个细菌，与小鼠生物测定法的相关性达到95.6%。

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本文引用的文献

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