Department of Health Sciences, Faculty of Medicine and Surgery, University of Molise, Campobasso, Italy.
J Microbiol Methods. 2011 Oct;87(1):119-24. doi: 10.1016/j.mimet.2011.07.011. Epub 2011 Jul 23.
Fifty Listeria monocytogenes strains were genotyped by sAFLP and PCR products were separated by agarose gel and automated chip-based microfluidic electrophoresis. A high congruency of results was observed comparing the two techniques, although for some cultures a better separation of sAFLP fragments was achieved with microfluidic system, which proved to be a highly reliable and reproducible tool to improve the molecular typing of L. monocytogenes, requiring lower volumes of samples and reducing significantly analysis time.
采用 sAFLP 对 50 株李斯特菌进行基因分型，琼脂糖凝胶和自动化芯片基微流控电泳分离 PCR 产物。两种技术的结果高度一致，尽管对于某些培养物，微流控系统可以更好地分离 sAFLP 片段，事实证明，该系统是一种非常可靠且可重复的工具，可提高李斯特菌的分子分型水平，所需的样品量更少，分析时间也大大缩短。
