克隆分组法，一种用于鉴定单核细胞增生李斯特菌主要克隆的快速多重聚合酶链反应方法。
Clonogrouping, a Rapid Multiplex PCR Method for Identification of Major Clones of Listeria monocytogenes.
作者信息
Chenal-Francisque Viviane, Maury Mylène M, Lavina Morgane, Touchon Marie, Leclercq Alexandre, Lecuit Marc, Brisse Sylvain
机构信息
Institut Pasteur, Biology of Infection Unit, National Reference Centre and WHO Collaborating Centre for Listeria, Paris, France.
Institut Pasteur, Microbial Evolutionary Genomics, Paris, France CNRS, UMR 3525, Paris, France Paris Diderot University, Sorbonne Paris Cité, Cellule Pasteur, Paris, France.
出版信息
J Clin Microbiol. 2015 Oct;53(10):3355-8. doi: 10.1128/JCM.00738-15. Epub 2015 Jul 29.
Abstract
Three multiplex PCR assays were developed to identify the 11 most common Listeria monocytogenes clones in clinical and food samples; 270 (95.7%) of 282 strains of serogroups IVb, IIb, IIa, and IIc were identified accurately. This novel tool is a rapid and efficient alternative to multilocus sequence typing for identification of L. monocytogenes clones.
摘要
开发了三种多重PCR检测方法，以鉴定临床和食品样本中11种最常见的单核细胞增生李斯特菌克隆；282株血清群IVb、IIb、IIa和IIc菌株中的270株（95.7%）被准确鉴定。这种新工具是用于鉴定单核细胞增生李斯特菌克隆的多位点序列分型的快速有效替代方法。
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