开发一种 ELISA 程序来研究莠去津在添加污水污泥的淋溶土中的吸附。

Development of an ELISA procedure to study sorption of atrazine onto a sewage sludge-amended luvisol soil.

机构信息

CESAM & Department of Chemistry, University of Aveiro, Aveiro, Portugal.

出版信息

Talanta. 2011 Sep 15;85(3):1494-9. doi: 10.1016/j.talanta.2011.06.024. Epub 2011 Jun 17.

DOI:10.1016/j.talanta.2011.06.024

Abstract

Pesticides may contaminate ground and surface waters and one of the major factors governing this property is soil sorption. Sorption can be assessed by batch equilibrium technique which produces lots of extracts with high dissolved organic carbon concentration in which the pesticide concentration has to be determined. We developed an ELISA procedure to analyse atrazine based on polyclonal antibodies (C193) for which tracer structure and dilutions of immunochemical reagents were adapted to fit the purpose. After a 1000-fold dilution (or after an SPE clean-up procedure) extracts of a sewage-sludge amended luvisol (used as an example application of the methodology developed) could be reliably analysed. The Freundlich model is able to describe adsorption for this system (r(2)=0.977) delivering a distribution coefficient K(F) of 1.6±0.2 (mg kg(-1)) (mg L(-1))(-N) and an isotherm nonlinearity factor N of 0.70±0.09.

摘要

农药可能会污染地下水和地表水，而控制这一特性的主要因素之一是土壤吸附。吸附可以通过批量平衡技术来评估，该技术会产生大量含有高浓度溶解有机碳的提取物，需要在其中测定农药浓度。我们开发了一种 ELISA 方法来分析莠去津，该方法基于多克隆抗体（C193），针对其示踪剂结构和免疫化学试剂的稀释度进行了调整，以适应目的。经过 1000 倍稀释（或 SPE 净化程序后），可以可靠地分析经过污水污泥改良的淋溶土（用作所开发方法的应用示例）的提取物。对于该体系，Freundlich 模型能够描述吸附情况（r(2)=0.977），提供分配系数 K(F)为 1.6±0.2（mg kg(-1))(mg L(-1))(-N)，等温非线性因子 N 为 0.70±0.09。