CESAM & Department of Chemistry, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal.
Environ Sci Pollut Res Int. 2013 May;20(5):3157-64. doi: 10.1007/s11356-012-1227-z. Epub 2012 Oct 7.
The implementation of the Water Framework Directive (2000/60/EC) requires the establishment of monitoring programs. However, conventional procedures for sample preparation prior to chromatographic analysis are rather expensive and time consuming, being the development of cost-effective and easy tool a necessity. The aim of this work was to develop an enzyme-linked immunosorbent assay (ELISA) able to determine atrazine in water samples. Matrix effects evaluation showed that the increase of humic acid (HA) concentration leads to flattened calibration curves and to the loss of the sigmoidal shape. However, such interference was overcome, by the presence of an environmental sample buffer, incubated together with the samples. Recoveries from 88.5 to 119.2 % were obtained in the presence of HA concentrations up to 20 mg L(-1). An analytical range from 0.003 to 1 μg L(-1) was obtained, and atrazine was detected in a sewage treatment plant with concentrations ranging from 14 to 52 ng L(-1).
《水框架指令（2000/60/EC）》的实施要求建立监测计划。然而，色谱分析前的常规样品制备程序相当昂贵且耗时，因此开发具有成本效益和易于使用的工具是必要的。本工作旨在开发一种能够测定水样中莠去津的酶联免疫吸附测定法（ELISA）。基质效应评估表明，腐殖酸（HA）浓度的增加会导致校准曲线变平，并失去钟形曲线的形状。然而，通过存在环境样品缓冲液，可以克服这种干扰，该缓冲液与样品一起孵育。在 HA 浓度高达 20 mg L(-1)时，回收率为 88.5%至 119.2%。获得了 0.003 至 1 μg L(-1)的分析范围，并在一个污水处理厂检测到莠去津，浓度范围为 14 至 52 ng L(-1)。
