Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at University of California, Los Angeles, CA 90095, USA.
Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):13700-4. doi: 10.1073/pnas.1107172108. Epub 2011 Aug 1.
B-1 B cells have been proposed to be preferentially generated from fetal progenitors, but this view is challenged by studies concluding that B-1 production is sustained throughout adult life. To address this controversy, we compared the efficiency with which hematopoietic stem cells (HSCs) and common lymphoid progenitors (CLPs) from neonates and adults generated B-1 cells in vivo and developed a clonal in vitro assay to quantify B-1 progenitor production from CLPs. Adult HSCs and CLPs generated fewer B-1 cells in vivo compared with their neonatal counterparts, a finding corroborated by the clonal studies that showed that the CLP compartment includes B-1- and B-2-specified subpopulations and that the former cells decrease in number after birth. Together, these data indicate that B-1 lymphopoiesis is not sustained at constant levels throughout life and define a heretofore unappreciated developmental heterogeneity within the CLP compartment.
B-1 细胞被认为优先由胎儿祖细胞产生，但这一观点受到了一些研究的挑战，这些研究得出的结论是 B-1 细胞的产生贯穿成年期。为了解决这一争议，我们比较了新生儿和成年造血干细胞 (HSCs) 和普通淋巴祖细胞 (CLPs) 在体内生成 B-1 细胞的效率，并开发了一种克隆体外测定法来定量从 CLPs 生成 B-1 祖细胞的情况。与新生儿相比，成年 HSCs 和 CLPs 在体内生成的 B-1 细胞较少，这一发现得到了克隆研究的证实，该研究表明 CLP 区室包括 B-1 和 B-2 特化的亚群，并且前者细胞在出生后数量减少。这些数据表明，B-1 淋巴生成并非在整个生命过程中保持恒定水平，并且定义了迄今为止在 CLP 区室中尚未被认识到的发育异质性。
