大鼠和小鼠中肠毒素型大肠杆菌热稳定毒素b的检测
Assay for enterotoxigenic Escherichia coli heat-stable toxin b in rats and mice.
作者信息
Whipp S C
机构信息
National Animal Disease Center, U.S. Department of Agriculture, Ames, Iowa 50010.
出版信息
Infect Immun. 1990 Apr;58(4):930-4. doi: 10.1128/iai.58.4.930-934.1990.
Abstract
The Escherichia coli heat-stable enterotoxin STb is the most prevalent toxin associated with diarrheagenic isolates of porcine origin. This report demonstrates that when endogenous protease activity was blocked with soybean trypsin inhibitor, STb evoked a dose-dependent secretory response in infant mice and jejunal loops of rats. Infant mice were much less sensitive to STb than rats were. The response of rat jejunal loops to STb was linearly related to the log of the dose of STb through 10 twofold dilutions. The anterior 25% of the jejunum was less sensitive than was the remainder of the gut. An incubation period of 2.5 h provided a maximal response. The linear response to STb in rats could be used as a bioassay.
摘要
大肠杆菌热稳定肠毒素STb是与猪源腹泻性分离株相关的最普遍毒素。本报告表明，当用大豆胰蛋白酶抑制剂阻断内源性蛋白酶活性时，STb在幼鼠和大鼠空肠肠袢中引发了剂量依赖性分泌反应。幼鼠对STb的敏感性远低于大鼠。大鼠空肠肠袢对STb的反应与STb剂量的对数呈线性关系，稀释倍数达10倍，共稀释10次。空肠前25%比肠道其余部分敏感性低。2.5小时的孵育期可产生最大反应。大鼠对STb的线性反应可用作生物测定。
相似文献
1
Assay for enterotoxigenic Escherichia coli heat-stable toxin b in rats and mice.大鼠和小鼠中肠毒素型大肠杆菌热稳定毒素b的检测
Infect Immun. 1990 Apr;58(4):930-4. doi: 10.1128/iai.58.4.930-934.1990.
2
Intestinal responses to enterotoxigenic Escherichia coli heat-stable toxin b in non-porcine species.非猪类物种对产肠毒素大肠杆菌热稳定毒素b的肠道反应。
Am J Vet Res. 1991 May;52(5):734-7.
3
Protease degradation of Escherichia coli heat-stable, mouse-negative, pig-positive enterotoxin.大肠杆菌热稳定、对小鼠无活性、对猪有活性的肠毒素的蛋白酶降解作用
Infect Immun. 1987 Sep;55(9):2057-60. doi: 10.1128/iai.55.9.2057-2060.1987.
4
Inverse relationship between heat stable enterotoxin-b induced fluid accumulation and adherence of F4ac-positive enterotoxigenic Escherichia coli in ligated jejunal loops of F4ab/ac fimbria receptor-positive swine.热稳定肠毒素-B 诱导的液体蓄积与 F4ab/ac 菌毛受体阳性猪结扎空肠袢中 F4ac 阳性肠产毒大肠杆菌黏附呈负相关。
Vet Microbiol. 2013 Jan 25;161(3-4):315-24. doi: 10.1016/j.vetmic.2012.07.048. Epub 2012 Aug 3.
5
6
Expression of heat-stable enterotoxin STb by adherent Escherichia coli is not sufficient to cause severe diarrhea in neonatal pigs.黏附性大肠杆菌表达的热稳定肠毒素STb不足以导致新生仔猪严重腹泻。
Infect Immun. 1998 Mar;66(3):1270-2. doi: 10.1128/IAI.66.3.1270-1272.1998.
7
Functional significance of histologic alterations induced by Escherichia coli pig-specific, mouse-negative, heat-stable enterotoxin (STb).猪特异性、小鼠阴性、热稳定肠毒素（STb）诱导的组织学改变的功能意义。
Vet Res Commun. 1987;11(1):41-55. doi: 10.1007/BF00361325.
8
The jejunal secretory response to Escherichia coli heat-stable enterotoxin is prolonged in malnourished rats.营养不良的大鼠对大肠杆菌热稳定肠毒素的空肠分泌反应会延长。
Pediatr Res. 1992 Mar;31(3):228-33. doi: 10.1203/00006450-199203000-00007.
9
Trypan blue uptake by chinese hamster ovary cultured epithelial cells: a cellular model to study Escherichia coli STb enterotoxin.中国仓鼠卵巢培养上皮细胞对锥虫蓝的摄取：一种研究大肠杆菌STb肠毒素的细胞模型。
Toxicon. 2002 Feb;40(2):185-91. doi: 10.1016/s0041-0101(01)00222-7.
10
Jejunal toxin inactivation regulates susceptibility of the immature rat to STa.空肠毒素失活调节未成熟大鼠对STa的易感性。
Gastroenterology. 1992 Jun;102(6):1988-96. doi: 10.1016/0016-5085(92)90324-r.
引用本文的文献
1
Enterotoxigenic Heat-Stable Toxin and Ebola Virus Delta Peptide: Similarities and Differences.产肠毒素热稳定毒素与埃博拉病毒δ肽：异同点
Pathogens. 2022 Jan 27;11(2):170. doi: 10.3390/pathogens11020170.
2
Interaction of Escherichia coli heat-stable enterotoxin B with cultured human intestinal epithelial cells.大肠杆菌热稳定肠毒素B与培养的人肠上皮细胞的相互作用。
Infect Immun. 1997 Aug;65(8):3209-17. doi: 10.1128/iai.65.8.3209-3217.1997.
3
Enteric bacterial toxins: mechanisms of action and linkage to intestinal secretion.肠道细菌毒素：作用机制及其与肠道分泌的联系
Microbiol Rev. 1996 Mar;60(1):167-215. doi: 10.1128/mr.60.1.167-215.1996.
4
Detection of toxin genes in Escherichia coli isolated from normal dogs and dogs with diarrhea.从正常犬和腹泻犬分离出的大肠杆菌中毒素基因的检测。
Can J Vet Res. 1995 Oct;59(4):265-70.
5
The structure of Escherichia coli heat-stable enterotoxin b by nuclear magnetic resonance and circular dichroism.通过核磁共振和圆二色性解析大肠杆菌热稳定肠毒素b的结构
Protein Sci. 1995 Sep;4(9):1718-29. doi: 10.1002/pro.5560040907.
6
Calcium influx mediated by the Escherichia coli heat-stable enterotoxin B (STB).由大肠杆菌热稳定肠毒素B（STB）介导的钙内流。
Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3202-6. doi: 10.1073/pnas.90.8.3202.
7
Effect of alterations of basic amino acid residues of Escherichia coli heat-stable enterotoxin II on enterotoxicity.大肠杆菌热稳定肠毒素II碱性氨基酸残基改变对肠毒性的影响。
Infect Immun. 1994 Jun;62(6):2295-301. doi: 10.1128/iai.62.6.2295-2301.1994.
8
Involvement of 5-hydroxytryptamine and prostaglandin E2 in the intestinal secretory action of Escherichia coli heat-stable enterotoxin B.5-羟色胺和前列腺素E2参与大肠杆菌热稳定肠毒素B的肠道分泌作用。
Infect Immun. 1995 Mar;63(3):745-50. doi: 10.1128/iai.63.3.745-750.1995.
9
Disulfide bond formation and secretion of Escherichia coli heat-stable enterotoxin II.大肠杆菌热稳定肠毒素II的二硫键形成与分泌
J Bacteriol. 1995 Aug;177(16):4579-86. doi: 10.1128/jb.177.16.4579-4586.1995.
10
Contribution of individual disulfide bonds to biological action of Escherichia coli heat-stable enterotoxin B.单个二硫键对大肠杆菌热稳定肠毒素B生物学活性的贡献。
Infect Immun. 1995 Dec;63(12):4715-20. doi: 10.1128/iai.63.12.4715-4720.1995.
本文引用的文献
1
Comparison of different assays for definition of heat-stable enterotoxigenicity of Escherichia coli porcine strains.不同检测方法对猪源大肠杆菌热稳定肠毒素产生能力定义的比较
J Clin Microbiol. 1980 Jan;11(1):6-15. doi: 10.1128/jcm.11.1.6-15.1980.
2
Characterization of the gene encoding heat-stable toxin II and preliminary molecular epidemiological studies of enterotoxigenic Escherichia coli heat-stable toxin II producers.产肠毒素大肠杆菌热稳定毒素II基因的特性分析及初步分子流行病学研究
Infect Immun. 1983 Oct;42(1):264-8. doi: 10.1128/iai.42.1.264-268.1983.
3
Effects of Escherichia coli heat-stable enterotoxin STb on intestines of mice, rats, rabbits, and piglets.大肠杆菌热稳定肠毒素STb对小鼠、大鼠、家兔和仔猪肠道的影响。
Infect Immun. 1984 Dec;46(3):639-43. doi: 10.1128/iai.46.3.639-643.1984.
4
Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu.利用幼鼠检测大肠杆菌肠毒素：在檀香山儿童腹泻研究中的应用
J Infect Dis. 1972 Apr;125(4):407-11. doi: 10.1093/infdis/125.4.407.
5
Functional significance of histologic alterations induced by Escherichia coli pig-specific, mouse-negative, heat-stable enterotoxin (STb).猪特异性、小鼠阴性、热稳定肠毒素（STb）诱导的组织学改变的功能意义。
Vet Res Commun. 1987;11(1):41-55. doi: 10.1007/BF00361325.
6
Effects of Escherichia coli heat-stable enterotoxin b on small intestinal villi in pigs, rabbits, and lambs.大肠杆菌热稳定肠毒素b对猪、兔和羔羊小肠绒毛的影响。
Vet Pathol. 1987 Jan;24(1):71-9. doi: 10.1177/030098588702400112.
7
Comparative prevalence of four enterotoxin genes among Escherichia coli isolated from swine.从猪中分离出的大肠杆菌中四种肠毒素基因的比较流行率。
Am J Vet Res. 1986 Feb;47(2):210-2.
8
Protease degradation of Escherichia coli heat-stable, mouse-negative, pig-positive enterotoxin.大肠杆菌热稳定、对小鼠无活性、对猪有活性的肠毒素的蛋白酶降解作用
Infect Immun. 1987 Sep;55(9):2057-60. doi: 10.1128/iai.55.9.2057-2060.1987.
9
Detection of enterotoxigenic Escherichia coli in piggeries in Victoria by DNA hybridisation using K88, K99, LT, ST1 and ST2 probes.利用K88、K99、LT、ST1和ST2探针通过DNA杂交技术检测维多利亚州养猪场中的产肠毒素大肠杆菌
Vet Microbiol. 1988 Mar;16(3):273-81. doi: 10.1016/0378-1135(88)90031-4.
10
Enzyme-linked immunosorbent assay for Escherichia coli heat-stable enterotoxin type II.II型大肠杆菌热稳定肠毒素的酶联免疫吸附测定
J Clin Microbiol. 1988 Aug;26(8):1555-60. doi: 10.1128/jcm.26.8.1555-1560.1988.
Zotero 插件