Monckton R P, Hasse D
Department of Agriculture and Rural Affairs, Regional Veterinary Laboratory, Bendigo, Victoria, Australia.
Vet Microbiol. 1988 Mar;16(3):273-81. doi: 10.1016/0378-1135(88)90031-4.
Rectal swabs collected from piglets with diarrhoea from commercial pig farms were examined for the presence of enterotoxigenic Escherichia coli (ETEC) using DNA hybridisation methods. The probes specifically detected genes for the K88 and K99 fimbrial antigens and the heat-labile and heat-stable enterotoxins. DNA hybridisation methods detected more ETEC than could be detected by either enzyme-linked immunosorbent assay (ELISA) or slide agglutination methods, and also offered the opportunity to test for fimbrial antigens and toxins concurrently. The DNA hybridization method was shown to be applicable to ETEC detection in mixed growths cultured directly from rectal swabs to filters. The method eliminates the need for toxin tests using animals and enables very large numbers of samples to be investigated. The use of toxin probes has revealed large numbers of ETEC with uncharacterized fimbrial antigens.
采用DNA杂交方法对从商业养猪场患有腹泻的仔猪采集的直肠拭子进行检查，以检测产肠毒素大肠杆菌（ETEC）的存在。这些探针能特异性检测K88和K99菌毛抗原以及热不稳定和热稳定肠毒素的基因。DNA杂交方法检测到的ETEC比酶联免疫吸附测定（ELISA）或玻片凝集法检测到的更多，并且还提供了同时检测菌毛抗原和毒素的机会。结果表明，DNA杂交方法适用于直接从直肠拭子培养到滤膜上的混合培养物中的ETEC检测。该方法无需使用动物进行毒素检测，并且能够检测大量样本。毒素探针的使用揭示了大量具有未鉴定菌毛抗原的ETEC。
