Integrative Oncology Department-Imaging Unit, British Columbia Cancer Agency Research Centre, Vancouver, British Columbia V5Z 1L3, Canada.
Opt Lett. 2011 Aug 1;36(15):2865-7. doi: 10.1364/OL.36.002865.
We present a multiphoton microscopy instrument specially designed for in vivo dermatological use that is capable of imaging human skin at 27 frames per second with 256 pixels × 256 pixels resolution without the use of exogenous contrast agents. Imaging at fast frame rates is critical to reducing image blurring due to patient motion and to providing practically short clinical measurement times. Second harmonic generation and two-photon fluorescence images and videos acquired at optimized wavelengths are presented showing cellular and tissue structures from the skin surface down to the reticular dermis.
我们展示了一种专门设计用于体内皮肤科应用的多光子显微镜仪器，该仪器能够以 27 帧/秒的速度、256 像素×256 像素的分辨率对人体皮肤进行成像，而无需使用外源性对比剂。高速帧率成像对于减少由于患者运动导致的图像模糊以及提供实际较短的临床测量时间至关重要。优化波长下获取的二次谐波产生和双光子荧光图像和视频显示了从皮肤表面到网状真皮的细胞和组织结构。
