Department of Physics, National Taiwan University, Taipei 106, Taiwan.
J Dermatol Sci. 2012 Mar;65(3):189-95. doi: 10.1016/j.jdermsci.2011.12.012. Epub 2011 Dec 24.
Tissue glycation, the main cause of many diabetes-related complications, results in the accumulation of advanced glycation endproducts (AGE).
These AGEs are endogenous fluorophores that can serve as a viable pathological indicator for disease diagnostics. Here we explore the capabilities of multiphoton microscopy to non-invasively localize and quantify the skin glycation.
In our study, multiphoton microscopy and spectroscopy were used to investigate glycation events-induced changes in the intensities of autofluorescence and second harmonic generation on ex vivo human skin.
Temporal and spatial dependence of degrees of glycation of the epidermis, collagen and elastin fibers of dermis were evaluated for their relevance to the changes in amplitudes of autofluorescence signals. We found that glycation drastically and linearly increases multiphoton autofluorescence intensity of epidermis and dermal collagen whereas changes in dermal elastin are moderate. We also found decrease in the level of second harmonic generation signal.
Our study suggests that due to intrinsically weak autofluorescence the dermal collagen is the most sensitive skin tissue to be used for detecting changes in tissue glycation.
组织糖基化是许多与糖尿病相关并发症的主要原因，会导致晚期糖基化终产物（AGE）的积累。
这些 AGE 是内源性荧光团，可以作为疾病诊断的可行病理指标。在这里，我们探讨了多光子显微镜在非侵入性定位和定量皮肤糖化方面的能力。
在我们的研究中，使用多光子显微镜和光谱学来研究糖基化事件诱导的离体人皮肤自发荧光和二次谐波产生强度的变化。
评估了表皮、真皮胶原和弹性纤维糖化程度的时空依赖性与自发荧光信号幅度变化的相关性。我们发现，糖基化使表皮和真皮胶原的多光子自发荧光强度急剧且线性增加，而真皮弹性蛋白的变化则适中。我们还发现二次谐波产生信号水平下降。
我们的研究表明，由于真皮胶原的自发荧光强度较弱，因此它是检测组织糖基化变化最敏感的皮肤组织。
