Department of Dermatology, University Hospital Jena, Jena, Germany.
Skin Res Technol. 2011 Nov;17(4):479-86. doi: 10.1111/j.1600-0846.2011.00522.x. Epub 2011 Mar 31.
Multiphoton tomography (MPT) is a novel non-invasive imaging method in dermatology allowing the depiction of the epidermis with sub-cellular resolution. Here, we present a descriptive characterization of unaffected human epidermis, morphometric data on human keratinocytes and some epidermal parameters in vivo and a morphological characterization of keratinocyte changes in actinic keratoses.
In a clinical setting, 57 volunteers of different age groups were examined using MPT.
The morphological appearance of keratinocytes showed polygonal cells in the horny layer, a granular cytoplasm in the stratum granulosum, smaller prickle cells in the stratum spinosum and hyperpigmented small round basal cells. Actinic keratoses presented remarkable differences including widened inter-cellular spaces, heterogeneity in cellular fluorescence and shape as well as an increased ratio of nuclear to cellular size. Finally, the thickness of the epidermis was significantly increased in actinic keratoses compared with the control.
In vivo MPT provides high-resolution images allowing the identification and quantification of cellular morphometric parameters. First observations of morphology and morphometry of actinic keratoses are reported.
多光子断层扫描（MPT）是一种新型的非侵入性皮肤科成像方法，可实现亚细胞分辨率的表皮描绘。在此，我们对正常的人类表皮进行了描述性特征分析，对人体角质形成细胞进行了计量学数据分析，并对活体表皮的一些参数进行了描述，还对光化性角化病中的角质形成细胞变化进行了形态学特征分析。
在临床环境中，使用 MPT 对 57 名不同年龄组的志愿者进行了检查。
角质形成细胞的形态外观显示在角质层中为多边形细胞，在颗粒层中为颗粒状细胞质，在棘层中为更小的刺状细胞，在基底细胞中有色素沉着的小圆基底细胞。光化性角化病表现出明显的差异，包括细胞间空间变宽、细胞荧光和形状的异质性以及核/细胞大小比增加。最后，与对照组相比，光化性角化病的表皮厚度明显增加。
体内 MPT 提供了高分辨率图像，可用于识别和量化细胞形态计量学参数。首次观察到了光化性角化病的形态和形态计量学特征。
