Nie Yingjie, Lin Pengcheng
College of Chemistry and Life Sciences, Qinghai University for Nationalities, Xi'ning 810000, China.
Zhongguo Zhong Yao Za Zhi. 2010 May;35(10):1276-9. doi: 10.4268/cjcmm20101012.
A new RP-HPLC method was developed for the simultaneous determination of 5 active constituents, including loganic acid, swertiamarin, gentiopicroside, sweroside, isoorientin in aerial part of Gentiana straminea. Analysis was achieved on a Hypersil ODS analytical column (4.6 mm x 250 mm, 5 microm) eluted with methanol and water containing 0.02% phosphoric acid in gradient elution. The flow rate was 1 mL x min(-1), the column temperature was set at 35 degrees C and detection wavelength was set at 254 nm. The results showed that 5 active components were separated well and showed good linearity. The corelation coefficints and concentration ranges of the calibration curves were as follow: r = 0.999 9, 0.364-2.18 microg for loganic acid. r = 0.999 9, 0.275-1.65 (microg for swertiamarin. r = 0.999 9, 0.614-3.68 microg for gentiopicroside. r = 0.999 9, 0.065 6-0.394 microg for sweroside. r = 0.999 9, 0.089 9-0.539 microg for isoorientin. The contents of loganic acid and gentiopicroside far exceed that of the other 3 active components, and made up 60% in the total contents. The developed method was proved to be rapid, sensitive, accurate, credible and repeatable. It can be applied to quality control of aerial part of Tibetan medicine Gentiana straminea.
建立了一种反相高效液相色谱（RP-HPLC）新方法，用于同时测定麻花艽地上部分的5种活性成分，包括马钱子酸、獐牙菜苦苷、龙胆苦苷、獐牙菜苷、异荭草苷。分析在Hypersil ODS分析柱（4.6 mm×250 mm，5μm）上进行，以甲醇和含0.02%磷酸的水进行梯度洗脱。流速为1 mL·min⁻¹，柱温设定为35℃，检测波长设定为254 nm。结果表明，5种活性成分分离良好，线性关系良好。校准曲线的相关系数和浓度范围如下：马钱子酸，r = 0.999 9，0.364 - 2.18μg；獐牙菜苦苷，r = 0.999 9，0.275 - 1.65μg；龙胆苦苷，r = 0.999 9，0.614 - 3.68μg；獐牙菜苷，r = 0.999 9，0.065 6 - 0.394μg；异荭草苷，r = 0.999 9，0.089 9 - 0.539μg。马钱子酸和龙胆苦苷的含量远高于其他3种活性成分，占总含量的60%。所建立的方法快速、灵敏、准确、可靠且可重复。可应用于藏药麻花艽地上部分的质量控制。
