Institute of Cytology and Genetics, 10, Lavrentieva ave, 630090, Novosibirsk, Russia.
Transgenic Res. 2012 Apr;21(2):429-37. doi: 10.1007/s11248-011-9538-2. Epub 2011 Aug 3.
The experiments with transgenic plants frequently demand selection of promoters providing appropriate transcription patterns. The set of promoters commonly used in vectors and genetic constructs is very limited, and these promoters provide only a few variants of gene expression patterns. Moreover, identical promoters in a complex construct can induce transgene silencing. This problem can be solved using a variety of plant gene promoters with experimentally verified characteristics. However, this requires a time-consuming analysis of literature data. Here, we describe a database of plant promoters (TransGene Promoters, TGP; http://wwwmgs.bionet.nsc.ru/mgs/dbases/tgp/home.html ). TGP contains the information on genomic DNA segments providing certain expression patterns of reporter genes in experiments with transgenic plants. TGP was constructed on the SRS platform, and its interface allows users to search for the promoters with particular characteristics.
转基因植物实验通常需要选择提供适当转录模式的启动子。载体和遗传构建物中常用的启动子集合非常有限，并且这些启动子仅提供几种基因表达模式的变体。此外，在复杂构建体中的相同启动子可能会诱导转基因沉默。可以使用具有经过实验验证特性的各种植物基因启动子来解决此问题。但是，这需要耗时的文献数据分析。在这里，我们描述了一个植物启动子数据库（转基因启动子，TGP; http://wwwmgs.bionet.nsc.ru/mgs/dbases/tgp/home.html）。TGP 包含在转基因植物实验中为报告基因提供特定表达模式的基因组 DNA 片段的信息。TGP 是在 SRS 平台上构建的，其界面允许用户搜索具有特定特征的启动子。
