Rahman Mohammed M, Alam M M, Asiri Abdullah M
Chemistry Department, Faculty of Science, King Abdulaziz University Jeddah 21589 P.O. Box 80203 Saudi Arabia.
Department of Chemical Engineering and Polymer Science, Shahjalal University of Science and Technology Sylhet 3100 Bangladesh
RSC Adv. 2019 Oct 31;9(60):35146-35157. doi: 10.1039/c9ra07459g. eCollection 2019 Oct 28.
In this study, a novel choline sensor was assembled by attaching the binary MnO/NiO nanoparticles (NPs) onto a glassy carbon electrode (GCE). Initially, MnO/NiO NPs were synthesized a wet-chemical process and fully characterized XRD, XPS, FESEM, EDS, FTIR and UV-Vis methods. The analytical performances of the choline sensor were evaluated by an electrochemical method in the phosphate buffer phase. The estimated linear dynamic range (LDR) was found to be 0.1 nM to 0.1 mM. The other analytical performances of the choline sensor, such as sensitivity (16.4557 μA μM cm) and detection limit (5.77 ± 0.29 pM), were also calculated very carefully from the calibration plot. Overall, the choline sensor exhibited a reliable reproducibility, validity, selectivity, interference effect, stability, and intra-day and inter-day performances with high accuracy in a short response time. Moreover, the probe was successfully applied to detect choline in real human, mouse and rabbit serum. This fabrication route would be a novel approach for the detection of selective biochemical sensor in the healthcare and biomedical fields.
在本研究中，通过将二元MnO/NiO纳米颗粒（NPs）附着到玻碳电极（GCE）上组装了一种新型胆碱传感器。最初，MnO/NiO NPs通过湿化学法合成，并采用XRD、XPS、FESEM、EDS、FTIR和UV-Vis方法进行了全面表征。在磷酸盐缓冲相中通过电化学方法评估了胆碱传感器的分析性能。估计的线性动态范围（LDR）为0.1 nM至0.1 mM。还从校准图中非常仔细地计算了胆碱传感器的其他分析性能，如灵敏度（16.4557 μA μM cm）和检测限（5.77 ± 0.29 pM）。总体而言，胆碱传感器在短响应时间内具有可靠的重现性、有效性、选择性、干扰效应、稳定性以及日内和日间性能，且精度高。此外，该探针已成功应用于检测真实人、小鼠和兔血清中的胆碱。这种制备途径将成为医疗保健和生物医学领域中检测选择性生化传感器的一种新方法。
