Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého 1/3, 612 42 Brno, Czech Republic.
Parasitology. 2011 Sep;138(11):1369-71. doi: 10.1017/S0031182011001041. Epub 2011 Aug 8.
In the Czech Republic, sera from 551 clinically healthy adult slaughtered pigs (females, 6-8 months old) were collected during the first half of June in 2010. Sera were tested for Toxoplasma gondii-specific IgG antibodies by an enzyme-linked immunosorbent assay; samples with more than 50% S/P were considered as positive. The same samples were also analysed for Neospora caninum antibodies using a commercial competitive-inhibition enzyme-linked immunosorbent assay; samples with more than 30% inhibition were considered as positive. Antibodies against T. gondii were found in 198 pigs (36%) in all districts with prevalences ranging from 18% to 75%. Antibodies against N. caninum were found in 16 pigs (3%); positive animals were found in 4 districts with prevalences ranging from 1% to 20%. Indication of mixed infections (concurrent presence of both N. caninum and T. gondii antibodies) was found in 8 (1·5%) pigs. The results of our study indicate that pigs in the Czech Republic have a relatively high seroprevalence for T. gondii, while they have only a low seroprevalence for N. caninum. Therefore, natural infection with T. gondii seems to be very common in Czech pigs. It is the first evidence of N. caninum antibodies in pigs in the Czech Republic. These results complete data about N. caninum infection in pigs in Europe.
2010 年 6 月上半月，在捷克共和国，从 551 头临床健康的成年屠宰猪（雌性，6-8 个月大）中采集血清。通过酶联免疫吸附试验检测血清中弓形虫特异性 IgG 抗体；S/P 比值大于 50%的样本被认为是阳性。使用商业竞争抑制酶联免疫吸附试验分析相同的样本是否存在新孢子虫抗体；抑制率大于 30%的样本被认为是阳性。在所有地区的 198 头猪（36%）中均发现了针对弓形虫的抗体，流行率从 18%到 75%不等。在 16 头猪（3%）中发现了针对新孢子虫的抗体；阳性动物分布在 4 个地区，流行率从 1%到 20%不等。在 8 头猪（1.5%）中发现了混合感染（同时存在新孢子虫和弓形虫抗体）的迹象。本研究结果表明，捷克共和国的猪弓形虫血清阳性率相对较高，而新孢子虫血清阳性率较低。因此，弓形虫的自然感染在捷克猪中似乎非常普遍。这是捷克猪中新孢子虫抗体的首次证据。这些结果完善了欧洲猪新孢子虫感染的数据。
