同基因免疫后利用脾细胞-骨髓瘤杂交瘤产生的抗体识别小鼠精子发生的分化抗原。
Recognition of differentiation antigens of spermatogenesis in the mouse by using antibodies from spleen cell-myeloma hybrids after syngeneic immunization.
作者信息
Bechtol K B, Brown S C, Kennett R H
出版信息
Proc Natl Acad Sci U S A. 1979 Jan;76(1):363-7. doi: 10.1073/pnas.76.1.363.
Abstract
Two differentiation antigens of spermatogenesis in the mouse are defined by monoclonal antibodies from hybridomas produced between the myeloma P3-X63Ag8 and spleen lymphocytes immunized with syngeneic testis cells. These antigens are on testis cells, and not liver, kidney, brain, spleen, or whole ovary. They are species but not strain specific and trypsin sensitive but collagenase insensitive. On the basis of their appearance during the onset of spermatogenesis they appear to be differentiation antigens expressed during different but overlapping time windows during spermatogenesis.
摘要
用骨髓瘤P3-X63Ag8与经同基因睾丸细胞免疫的脾淋巴细胞产生的杂交瘤所分泌的单克隆抗体，鉴定出小鼠精子发生的两种分化抗原。这些抗原存在于睾丸细胞上，而不存在于肝脏、肾脏、大脑、脾脏或整个卵巢中。它们具有种属特异性而非品系特异性，对胰蛋白酶敏感但对胶原酶不敏感。根据它们在精子发生开始时的出现情况，它们似乎是在精子发生过程中不同但重叠的时间窗口表达的分化抗原。
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Recognition of differentiation antigens of spermatogenesis in the mouse by using antibodies from spleen cell-myeloma hybrids after syngeneic immunization.同基因免疫后利用脾细胞-骨髓瘤杂交瘤产生的抗体识别小鼠精子发生的分化抗原。
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