阿片类药物和脑啡肽可抑制小鼠神经母细胞瘤细胞系N4TG1中神经节苷脂和膜糖蛋白的合成。
Opiates and enkephalins inhibit synthesis of gangliosides and membrane glycoproteins in mouse neuroblastoma cell line N4TG1.
作者信息
Dawson G, McLawhon R, Miller R J
出版信息
Proc Natl Acad Sci U S A. 1979 Feb;76(2):605-9. doi: 10.1073/pnas.76.2.605.
When mouse neuroblastoma clonal cell line N4TG1 cells were cultured in the presence of opiates or enkephalins, in the range 10(-6)-10(-10) M for 24 hr, a dose-dependent inhibition of the incorporation of [3H]glucosamine and [14C]-galactose into sialoglycosphingolipids and glycoproteins was observed. The gangliosides most affected comigrated in thinlayer chromatographic systems with GM2 (GalNAc[AcNeu]-Gal-Glc-ceramide), GM1 (Gal-GalNAc[AcNeu]Gal-Glc-ceramide), and GDla (AcNeu-Gal-GalNAc[AcNeu]Gal-Glc-ceramide). The effects were stereospecific and naloxone-reversible. Polyacrylamide gel electrophoresis revealed that the synthesis of a large number of membrane glycoproteins was also stereospecifically inhibited. Synthesis of other proteins and glycoproteins, proteoglycans, DNA, and membrane phospholipids and the rate of cell division were not altered in any specific or stereospecific manner. Moreover, clonal cell lines (neuroblastomas and oligodendroglioma) and human skin fibroblasts, which do not possess opiate receptors, did not respond to opiates or enkephalins in a stereospecific manner.
当小鼠神经母细胞瘤克隆细胞系N4TG1细胞在10(-6)-10(-10)M的阿片类药物或脑啡肽存在下培养24小时时，观察到[3H]葡萄糖胺和[14C]半乳糖掺入唾液糖鞘脂和糖蛋白的过程呈剂量依赖性抑制。受影响最大的神经节苷脂在薄层色谱系统中与GM2（GalNAc[AcNeu]-Gal-Glc-神经酰胺）、GM1（Gal-GalNAc[AcNeu]Gal-Glc-神经酰胺）和GDla（AcNeu-Gal-GalNAc[AcNeu]Gal-Glc-神经酰胺）共迁移。这些作用具有立体特异性且可被纳洛酮逆转。聚丙烯酰胺凝胶电泳显示，大量膜糖蛋白的合成也受到立体特异性抑制。其他蛋白质、糖蛋白、蛋白聚糖、DNA和膜磷脂的合成以及细胞分裂速率均未以任何特异性或立体特异性方式改变。此外，不具有阿片受体的克隆细胞系（神经母细胞瘤和少突胶质细胞瘤）和人皮肤成纤维细胞对阿片类药物或脑啡肽没有立体特异性反应。
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