来自构巢曲霉的UDP-葡萄糖-4-差向异构酶的初步X射线晶体学研究。
Preliminary X-ray crystallographic studies of UDP-glucose-4-epimerase from Aspergillus nidulans.
作者信息
Dalrymple Sean A, Sheoran Inder, Kaminskyj Susan G W, Sanders David A R
机构信息
Department of Chemistry, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Aug 1;67(Pt 8):885-7. doi: 10.1107/S1744309111020914. Epub 2011 Jul 19.
UDP-glucose-4-epimerase (GALE) from Aspergillus nidulans was overexpressed in Escherichia coli, purified via His-tag affinity chromatography and cocrystallized with UDP-galactose using the microbatch method. The crystals diffracted to 2.4 Å resolution using synchrotron radiation on the Canadian Light Source 08ID-1 beamline. Examination of the data with d*TREK revealed nonmerohedral twinning, from which a single lattice was ultimately extracted for processing. The final space group was found to be C2, with unit-cell parameters a = 66.13, b = 119.15, c = 161.42 Å, β = 98.48°. An initial structure solution has been obtained via molecular replacement employing human GALE (PDB entry 1hzj) as a template model.
构巢曲霉的UDP-葡萄糖-4-表异构酶（GALE）在大肠杆菌中过表达，通过His标签亲和色谱法纯化，并使用微量分批法与UDP-半乳糖共结晶。使用加拿大光源08ID-1光束线的同步辐射，晶体衍射分辨率达到2.4 Å。用d*TREK检查数据时发现了非等轴孪晶，最终从中提取了单个晶格进行处理。最终发现空间群为C2，晶胞参数a = 66.13、b = 119.15、c = 161.42 Å，β = 98.48°。通过以人GALE（PDB条目1hzj）为模板模型的分子置换获得了初始结构解。
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