细菌光敏 LOV 蛋白的功能、结构和机制。
Function, structure and mechanism of bacterial photosensory LOV proteins.
机构信息
Department of Biochemistry and Molecular Biology, The University of Chicago, Illinois 60637, USA.
出版信息
Nat Rev Microbiol. 2011 Aug 8;9(10):713-23. doi: 10.1038/nrmicro2622.
Abstract
LOV (light, oxygen or voltage) domains are protein photosensors that are conserved in bacteria, archaea, plants and fungi, and detect blue light via a flavin cofactor. LOV domains are present in both chemotrophic and phototrophic bacterial species, in which they are found amino-terminally of signalling and regulatory domains such as sensor histidine kinases, diguanylate cyclases-phosphodiesterases, DNA-binding domains and regulators of RNA polymerase σ-factors. In this Review, we describe the current state of knowledge about the function of bacterial LOV proteins, the structural basis of LOV domain-mediated signal transduction, and the use of LOV domains as genetically encoded photoswitches in synthetic biology.
摘要
LOV（光、氧或电压）结构域是在细菌、古菌、植物和真菌中保守的蛋白质光感受器，通过黄素辅因子检测蓝光。LOV 结构域存在于化能营养菌和光能营养菌中，位于信号和调节结构域的氨基端，如传感器组氨酸激酶、双鸟苷酸环化酶-磷酸二酯酶、DNA 结合结构域和 RNA 聚合酶 σ 因子的调节剂。在这篇综述中，我们描述了细菌 LOV 蛋白功能的现有知识状态、LOV 结构域介导的信号转导的结构基础，以及 LOV 结构域作为遗传编码光开关在合成生物学中的应用。
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