用人单纯疱疹病毒DNA转染人淋巴母细胞。
Transfection of human lymphoblastoid cells with herpes simplex viral DNA.
作者信息
Miller G, Wertheim P, Wilson G, Robinson J, Geelen J L, van der Noordaa J, van der Eb A J
出版信息
Proc Natl Acad Sci U S A. 1979 Feb;76(2):949-53. doi: 10.1073/pnas.76.2.949.
Abstract
The "calcium/dimethyl sulfoxide shock" method of transfection was adapted for use in human lymphoid cell cultures. One microgram of herpes simplex virus type 1 DNA regularly initiated virus replication in four lymphoblastoid cell lines. Per 10(5) cells exposed to 1 microgram of DNA, 0.5--5 cells formed an infectious center. The minimal infective dose of DNA was approximately 500 ng.
摘要
“钙/二甲基亚砜休克”转染方法被应用于人类淋巴细胞培养。1微克1型单纯疱疹病毒DNA能在四种淋巴母细胞系中常规引发病毒复制。每10⁵个暴露于1微克DNA的细胞中，有0.5 - 5个细胞形成感染中心。DNA的最小感染剂量约为500纳克。
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