Laboratorio de Virología Molecular, CMBC, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela.
Nat Prod Res. 2011 Sep;25(16):1565-9. doi: 10.1080/14786419.2010.535164. Epub 2011 Aug 10.
The primary objective of this study was to search for natural products capable of inhibiting hepatitis B virus (HBV) replication. The research design, methods and procedures included testing hydro-alcoholic extracts (n = 66) of 31 species from the Venezuelan Amazonian rain forest on the cell line HepG2 2.2.15, which constitutively produces HBV. The main outcomes and results were as follows: the species Euterpe precatoria, Jacaranda copaia, Jacaranda obtusifolia, Senna silvestris, Warscewiczia coccinea and Vochysia glaberrima exerted some degree of inhibition on HBV replication. The leaves of W. coccinea showed a significant antiviral activity: 80% inhibition with 100 µg mL⁻¹ of extract. This extract also exerted inhibition on covalently closed circular deoxyribonucleic acid (cccDNA) production and on HIV-1 replication in MT4 cells (more than 90% inhibition with 50 µg mL⁻¹ of extract). Initial fractionation using organic solvents of increasing polarity and water showed that the ethanol fraction was responsible for most of the antiviral inhibitory activities of both the viruses. It was concluded that Warscewiczia coccinea extract showed inhibition of HBV and HIV-1 replication. Bioassay-guided purification of this fraction may allow the isolation of an antiviral compound with inhibitory activity against both viruses.
本研究的主要目的是寻找能够抑制乙型肝炎病毒 (HBV) 复制的天然产物。研究设计、方法和程序包括在 HepG2 2.2.15 细胞系上测试来自委内瑞拉亚马逊雨林的 31 种植物的水醇提取物 (n = 66)，该细胞系持续产生 HBV。主要结果如下：Euterpe precatoria、Jacaranda copaia、Jacaranda obtusifolia、Senna silvestris、Warscewiczia coccinea 和 Vochysia glaberrima 等物种对 HBV 复制有一定程度的抑制作用。W. coccinea 的叶子表现出显著的抗病毒活性：用 100μg/mL 的提取物可达到 80%的抑制率。该提取物还对共价闭合环状 DNA (cccDNA) 的产生和 MT4 细胞中的 HIV-1 复制具有抑制作用（用 50μg/mL 的提取物可达到超过 90%的抑制率）。使用极性逐渐增加的有机溶剂和水进行初步分级分离，结果表明乙醇级分是两种病毒大多数抗病毒抑制活性的主要来源。研究结果表明，Warscewiczia coccinea 提取物对 HBV 和 HIV-1 的复制具有抑制作用。对该部分进行基于生物测定的纯化可能会分离出一种对两种病毒均具有抑制活性的抗病毒化合物。
