[赤桉果实提取物抗单纯疱疹病毒1型和乙肝病毒活性的体外研究]
[In vitro study on the anti-HSV-1 and HBV activities of extracts from the fruit of Eucalyptus maidenii].
作者信息
Ju Huai-qiang, Wang Sha-yan, Pei Ying, Xiang Yang-fei, Li Shen, Zhang Ying-jun, Yang Chong-ren, Wang Yi-fei
机构信息
Shenzhen People's Hospital, Shenzhen 518020, China.
出版信息
Zhong Yao Cai. 2011 Feb;34(2):242-5.
OBJECTIVE
To investigate the antiviral activities of three kinds of extracts from the fruit of Eucalyptus maidenii against herpes simplex virus typel and Hepatitis B Virus.
METHODS
Cytotoxicity of extracts on Vero cell lines were estimated using MTT method and anti-HSV-1 activity was observed and determined with CPE and plaque reduction assay. The inhibitory effects of extracts on HBsAg and HBeAg secretion in HepG2.2.15 cell culture were detected using ELISA.
RESULTS
Aqueous extract (pl8-E3) had conspicuous anti-HSV-1 activity, the IC50 was 126.77 microg/mL,but the EtOAc extracts( pl8-E1 )and MeOH extracts (pl8-E2) showed little anti-HSV-1 activity. None of these extracts had significant inhibitory eflect on HBsAg and HBeAg secretion in HepG2.2.15 cell culture.
CONCLUSION
Aqueous extract(p18-E3) from the fruit of Eucalyptus maidenii has conspicuous anti-HSV-1 activity. It could inactivate virus directly,and inhibit virus attachment,but had no influence on virus penetration. The mechanism that p18-E3 inactivates virus might involve in viral envelope alteration.
目的
研究三种细叶桉果实提取物对单纯疱疹病毒1型和乙型肝炎病毒的抗病毒活性。
方法
采用MTT法测定提取物对Vero细胞系的细胞毒性，通过细胞病变效应（CPE）和空斑减少试验观察并测定抗单纯疱疹病毒1型活性。使用酶联免疫吸附测定（ELISA）检测提取物对HepG2.2.15细胞培养中乙肝表面抗原（HBsAg）和乙肝e抗原（HBeAg）分泌的抑制作用。
结果
水提取物（pl8-E3）具有显著的抗单纯疱疹病毒1型活性，半数抑制浓度（IC50）为126.77微克/毫升，但乙酸乙酯提取物（pl8-E1）和甲醇提取物（pl8-E2）显示出微弱的抗单纯疱疹病毒1型活性。这些提取物均对HepG2.2.15细胞培养中HBsAg和HBeAg的分泌没有显著抑制作用。
结论
细叶桉果实的水提取物（p18-E3）具有显著的抗单纯疱疹病毒1型活性。它可直接使病毒失活，并抑制病毒附着，但对病毒穿透没有影响。p18-E3使病毒失活的机制可能涉及病毒包膜改变。
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