Division of Research, Department of Transfusion Monitoring, Sanquin Blood Supply Foundation, Groningen, the Netherlands.
Transfusion. 2012 Mar;52(3):489-92. doi: 10.1111/j.1537-2995.2011.03298.x. Epub 2011 Aug 9.
During processing and storage, red blood cells (RBCs) undergo changes and cell injury resulting in hemolysis. Mostly, the separation of whole blood in top-and-bottom quadruple bag systems with break openings takes less than 4 minutes. However, longer separation times are not uncommon. The aims were to investigate whether hemolysis is increased in RBCs with longer separation time (RBCs(>6 min)) compared to regular RBCs (RBCs(reg)), to measure hemolysis increase during storage and to study whether frequency of hemolytic donations is donor dependent.
RBCs(>6 min) (n = 172) and 172 matched controls were tested for hemolysis on Days 1 and 21 RBC units from each group were stored at 4 ± 2°C and tested again after 5 weeks. Donor dependency was retrospectively investigated for 100 hemolytic RBC units.
RBCs(>6 min) exhibited a higher mean hemolysis rate than RBCs(reg) (0.058% vs. 0.033%). Four RBC units were hemolytic (>0.8%), all RBCs(>6 min) (2.36%). During storage, hemolysis in both groups increased with 0.24%. Hemolysis frequency did not seem to be donor dependent.
Increased separation time is a useful screening tool for potentially increased hemolysis rate in RBCs. Hemolysis rate increased during storage equally in both groups. Hemolysis frequency appears donor independent.
在处理和储存过程中，红细胞（RBC）会发生变化和细胞损伤，导致溶血。在上下四分袋系统中，全血的分离大多在 4 分钟内完成，但也有较长的分离时间。目的是研究与常规红细胞（RBCs(reg)）相比，分离时间较长（RBCs(>6 min)）的红细胞是否会增加溶血，测量储存过程中溶血的增加情况，并研究溶血献血的频率是否与供体有关。
对 172 例 RBCs(>6 min)和 172 例匹配对照进行第 1 天和第 21 天的溶血检测，对每组的 172 个 RBC 单位在 4±2°C 下储存，5 周后再次检测。对 100 个溶血 RBC 单位进行了供体依赖性的回顾性调查。
RBCs(>6 min)的平均溶血率高于 RBCs(reg)（0.058%比 0.033%）。有 4 个 RBC 单位发生溶血（>0.8%），均为 RBCs(>6 min)（2.36%）。在储存过程中，两组的溶血率均增加了 0.24%。溶血频率似乎与供体无关。
分离时间延长是红细胞潜在溶血率升高的有用筛选工具。两组的溶血率在储存过程中都相等地增加。溶血频率似乎与供体无关。
