Department of Orthopaedics and Rehabilitation, University of Iowa Hospitals and Clinics, Iowa City, IA, USA.
Am J Sports Med. 2011 Nov;39(11):2355-61. doi: 10.1177/0363546511417172. Epub 2011 Aug 9.
The use of allogenic juvenile chondrocytes or autologous chondral fragments has shown promising laboratory results for the repair of chondral lesions.
Juvenile chondrocytes would not affect matrix production when mixed with adult chondrocytes or cartilage fragments.
Controlled laboratory study.
Cartilage sources consisted of 3 adult and 3 juvenile (human) donors. In part 1, per each donor, juvenile chondrocytes were mixed with adult chondrocytes in 5 different proportions: 100%, 50%, 25%, 12.5%, and 0%. Three-dimensional cultures in low-melt agarose were performed. At 6 weeks, biochemical and histologic analyses were performed. In part 2, isolated adult, isolated juvenile, and mixed 3-dimensional cultures (1:1) were performed with chondral fragments (<1 mm), both with low-melt agarose and a hyaluronic acid scaffold. At 2 and 6 weeks, cultures were evaluated with biochemical and histologic analyses.
Part 1: Biochemical and histologic analyses showed that isolated juvenile cultures performed significantly better than mixed and isolated adult cultures. No significant differences were noted between mixed cultures (1:1) and isolated adult cultures. Part 2: Biochemical and histologic results at 6 weeks showed that mixed cartilage fragment cultures performed better than isolated adult cultures in terms of proteoglycans/DNA ratio (P = .014), percentage of safranin O-positive cells (P = .012), Bern score (P = .001), and collagen type II. No statistically significant difference was noted between juvenile and mixed cultures.
Extracellular matrix production of juvenile chondrocytes is inhibited by adult chondrocytes. The addition of juvenile cartilage fragments to adult fragments improves matrix production, with a positive interaction between the 2 sources.
Even if the underlying mechanisms are still unknown, this study describes the behavior of juvenile/adult cocultures using both chondrocytes and cartilage fragments, with potential for new research and clinical applications.
使用同种异体的幼年软骨细胞或自体软骨碎片修复软骨损伤，在实验室中取得了有前景的结果。
当与成体软骨细胞或软骨碎片混合时，幼年软骨细胞不会影响基质的产生。
对照的实验室研究。
软骨来源于 3 名成人和 3 名（人类）青少年供体。在第 1 部分，对于每个供体，将幼年软骨细胞与成体软骨细胞以 5 种不同的比例混合：100%、50%、25%、12.5%和 0%。进行三维培养在低熔点琼脂糖中进行。在 6 周时，进行生化和组织学分析。在第 2 部分，用软骨碎片（<1 毫米）进行分离的成年、分离的青少年和混合的三维培养（1:1），两者均使用低熔点琼脂糖和透明质酸支架。在 2 周和 6 周时，通过生化和组织学分析评估培养物。
第 1 部分：生化和组织学分析表明，分离的幼年培养物的表现明显优于混合和分离的成年培养物。混合培养物（1:1）与分离的成年培养物之间没有显著差异。第 2 部分：6 周时的生化和组织学结果表明，混合软骨碎片培养物在糖胺聚糖/DNA 比（P =.014）、番红 O 阳性细胞百分比（P =.012）、Bern 评分（P =.001）和胶原蛋白 II 方面的表现优于分离的成年培养物。在幼年和混合培养物之间没有统计学上的显著差异。
成体软骨细胞抑制幼年软骨细胞细胞外基质的产生。向成年软骨碎片中添加幼年软骨碎片可改善基质的产生，两种来源之间存在积极的相互作用。
即使潜在的机制仍不清楚，本研究描述了使用软骨细胞和软骨碎片的幼年/成年共培养物的行为，为新的研究和临床应用提供了潜力。
