Smith H R, Scotland S M, Stokes N, Rowe B
Division of Enteric Pathogens, Central Public Health Laboratory, London.
J Med Microbiol. 1990 Apr;31(4):235-40. doi: 10.1099/00222615-31-4-235.
Four hundred and forty-nine strains isolated from patients with diarrhoea and belonging to 13 enteropathogenic Escherichia coli (EPEC) O serogroups were tested with a DNA probe for the EPEC adherence factor (EAF). Positive results were obtained with only 36 strains; they belonged to 10 O serogroups and flagellar typing showed they were usually of the "classical" EPEC serotypes. Thirty-four of the 36 EAF-positive strains showed localised adhesion to HEp-2 cells. The two remaining strains, of serotypes O114:H2 and O127:H4, showed low level or no adhesion to HEp-2 cells. No colonies hybridising with the EAF probe were identified in cultures from 115 faecal specimens from healthy children. Sixteen of the 449 strains hybridised with one or both probes for the Vero cytotoxin genes VT1 and VT2; 15 of the 16 strains belonged to serogroups O26 and O128. None of the strains hybridised with both the EAF and VT gene probes. These studies show that the great majority of strains belonging to EPEC O serogroups do not possess the EPEC adherence factor or carry VT genes.
从腹泻患者中分离出的449株属于13种肠致病性大肠杆菌（EPEC）O血清群的菌株，用EPEC黏附因子（EAF）的DNA探针进行检测。仅36株得到阳性结果；它们属于10个O血清群，鞭毛分型显示它们通常为“经典”EPEC血清型。36株EAF阳性菌株中的34株对HEp-2细胞表现出局部黏附。其余两株血清型为O114:H2和O127:H4的菌株，对HEp-2细胞表现出低水平黏附或无黏附。在来自健康儿童的115份粪便标本培养物中，未鉴定出与EAF探针杂交的菌落。449株菌株中有16株与Vero细胞毒素基因VT1和VT2的一种或两种探针杂交；16株中的15株属于O26和O128血清群。没有菌株同时与EAF和VT基因探针杂交。这些研究表明，属于EPEC O血清群的绝大多数菌株不具有EPEC黏附因子或携带VT基因。
