Lipid and Lipoprotein Studies, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, United States of America.
PLoS One. 2011;6(7):e22360. doi: 10.1371/journal.pone.0022360. Epub 2011 Jul 28.
The first goal of this study was to measure the oxidative stress (OS) and relate it to lipoprotein variables in 35 renal patients before dialysis (CKD), 37 on hemodialysis (HD) and 63 healthy subjects. The method for OS was based on the ratio of cholesteryl esters (CE) containing C18/C16 fatty acids (R2) measured by gas chromatography (GC) which is a simple, direct, rapid and reliable procedure. The second goal was to investigate and identify a triacylglycerol peak on GC, referred to as TG48 (48 represents the sum of the three fatty acids carbon chain lengths) which was markedly increased in renal patients compared to healthy controls. We measured TG48 in patients and controls. Mass spectrometry (MS) and MS twice in tandem were used to analyze the fatty acid composition of TG48. MS showed that TG48 was abundant in saturated fatty acids (SFAs) that were known for their pro-inflammatory property. TG48 was significantly and inversely correlated with OS. Renal patients were characterized by higher OS and inflammation than healthy subjects. Inflammation correlated strongly with TG, VLDL-cholesterol, apolipoprotein (apo) C-III and apoC-III bound to apoB-containing lipoproteins, but not with either total cholesterol or LDL-cholesterol.In conclusion, we have discovered a new inflammatory factor, TG48. It is characterized with TG rich in saturated fatty acids. Renal patients have increased TG48 than healthy controls.
本研究的首要目标是测量氧化应激（OS），并将其与 35 名透析前（CKD）、37 名血液透析（HD）和 63 名健康受试者的脂蛋白变量相关联。OS 的测定方法基于气相色谱（GC）测定的含有 C18/C16 脂肪酸的胆甾醇酯（CE）的比率（R2），这是一种简单、直接、快速和可靠的方法。第二个目标是研究并鉴定 GC 上的三酰甘油峰，称为 TG48（48 代表三个脂肪酸碳链长度的总和），与健康对照组相比，肾病患者中的 TG48 明显增加。我们测量了患者和对照组中的 TG48。采用质谱（MS）和串联质谱（MS/MS）两次分析 TG48 的脂肪酸组成。MS 表明，TG48 富含具有促炎特性的饱和脂肪酸（SFAs）。TG48 与 OS 呈显著负相关。与健康受试者相比，肾病患者具有更高的 OS 和炎症。炎症与 TG、VLDL-胆固醇、载脂蛋白（apo）C-III 和与载脂蛋白 B 结合的 apoC-III 强烈相关，但与总胆固醇或 LDL-胆固醇无关。总之，我们发现了一种新的炎症因子，即 TG48。它的特点是富含 TG 的饱和脂肪酸。与健康对照组相比，肾病患者的 TG48 增加。
