Department of Physiology and Biochemistry, Faculty of Rehabilitation, University School of Physical Education Kraków, al. Jana Pawla II 78, 31-571 Kraków, Poland.
Neurochem Res. 2012 Dec;37(12):2715-24. doi: 10.1007/s11064-012-0862-6. Epub 2012 Aug 19.
In the present study we have evaluated the effect of a single hemodialysis session on the brain-derived neurotrophic factor levels in plasma BDNF and in serum BDNF as well as on the plasma isoprostanes concentration F(2) isoprostanes, plasma total antioxidant capacity (TAC) and plasma cortisol levels in chronic kidney disease patients. Twenty male patients (age 69.8 ± 2.9 years (mean ± SE)) with end-stage renal disease undergoing maintenance hemodialysis on regular dialysis treatment for 15-71 months participated in this study. A single hemodialysis session, lasting 4.2 ± 0.1 h, resulted in a decrease (P = 0.014) in BDNF by ~42 % (2,574 ± 322 vs. 1,492 ± 327 pg ml(-1)). This was accompanied by an increase (P < 10(-4)) of F(2)-Isoprostanes (38 ± 3 vs. 116 ± 16 pg ml(-1)), decrease (P < 10(-4)) in TAC (1,483 ± 41 vs. 983 ± 35 trolox equivalents, μmol l(-1)) and a decrease (P = 0.004) in plasma cortisol level (449.5 ± 101.2 vs. 315.3 ± 196.3 nmol l(-1)). No changes (P > 0.05) in BDNF and the platelets count were observed after a single dialysis session. Furthermore, basal BDNF in the chronic kidney disease patients was significantly lower (P = 0.03) when compared to the age-matched control group (n = 23). We have concluded that the observed decrease in serum BDNF level after hemodialysis accompanied by elevated F(2)-Isoprostanes and decreased plasma TAC might be caused by enhanced oxidative stress induced by hemodialysis.
在本研究中，我们评估了单次血液透析对慢性肾脏病患者血浆脑源性神经营养因子（BDNF）BDNF和血清 BDNF BDNF水平、血浆异前列烷浓度F(2)异前列烷、血浆总抗氧化能力（TAC）和血浆皮质醇水平的影响。20 名男性患者（年龄 69.8 ± 2.9 岁（均值 ± SE））患有终末期肾病，在常规透析治疗下接受维持性血液透析治疗 15-71 个月。单次血液透析持续 4.2 ± 0.1 h，导致 BDNF降低（P = 0.014）约 42%（2,574 ± 322 对 1,492 ± 327 pg ml(-1)）。这伴随着 F(2)-异前列烷（38 ± 3 对 116 ± 16 pg ml(-1)）的增加（P < 10(-4)）、TAC（1,483 ± 41 对 983 ± 35 trolox 当量，μmol l(-1)）的减少（P < 10(-4)）和血浆皮质醇水平（449.5 ± 101.2 对 315.3 ± 196.3 nmol l(-1)）的降低（P = 0.004）。单次透析后，BDNF和血小板计数无变化（P > 0.05）。此外，与年龄匹配的对照组（n = 23）相比，慢性肾脏病患者的基础 BDNF水平显著降低（P = 0.03）。我们得出结论，血液透析后血清 BDNF 水平下降，同时 F(2)-异前列烷升高，血浆 TAC 降低，可能是血液透析引起的氧化应激增强所致。
