Department of Physics, University of Messina, Viale D'Alcontres 31, P.O. Box 55, 98166 Messina, Italy.
J Phys Chem B. 2011 Sep 22;115(37):11004-9. doi: 10.1021/jp205599a. Epub 2011 Aug 24.
In this work vibrational spectra of mixtures of two glass-forming bioprotectant systems, i.e., trehalose and glycerol, are collected at very low temperature by using the indirect geometry time-of-flight (t.o.f.) TOSCA spectrometer at the ISIS Pulse Neutron Facility (Rutherford Appleton Laboratory, Oxford, U.K.). The main aim of this work is to investigate, through inelastic neutron scattering (INS), the vibrational behavior of trehalose and its mixtures with glycerol at different concentration values in order to characterize the changes induced by glycerol on the trehalose hydrogen bonded network. The obtained experimental findings, which are discussed and interpreted in the framework of previous INS, quasi elastic neutron scattering (QENS) and molecular simulation data obtained on trehalose/glycerol mixtures at different concentration and temperature values, will be linked to the different mixtures bioprotectant effectiveness.
在这项工作中，使用间接几何飞行时间（TOF）TOSCA 光谱仪在 ISIS 脉冲中子设施（英国牛津卢瑟福·阿普尔顿实验室）中，在非常低的温度下收集了两种玻璃形成生物保护剂系统，即海藻糖和甘油的混合物的振动光谱。这项工作的主要目的是通过非弹性中子散射（INS）研究海藻糖及其与甘油的混合物在不同浓度值下的振动行为，以表征甘油对海藻糖氢键网络的影响。所得实验结果将在先前的 INS、准弹性中子散射（QENS）和在不同浓度和温度值下获得的海藻糖/甘油混合物的分子模拟数据的框架内进行讨论和解释，并与不同混合物的生物保护剂有效性联系起来。
