Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Tsushima, Okayama 700-8530, Japan.
Phytochemistry. 2011 Nov;72(16):1978-89. doi: 10.1016/j.phytochem.2011.07.011. Epub 2011 Aug 8.
Shoot cultures of Tamarix tetrandra on Linsmaier-Skoog (LS) agar medium with 30 g l(-1) sucrose, 2.13 mg l(-1) indoleacetic acid and 2.25 mg l(-1) benzyl adenine produced ellagitannins found in intact plants of the Tamaricaceae. This was demonstrated by the isolation of 14 monomeric-tetrameric ellagitannins from the aq. Me2CO extract of the cultured tissues. This is the first report on the production of ellagitannin tetramers by plant tissue culture. The effects of light and certain medium constituents on tissue growth and ellagitannin production were examined. The contents of representative tannins of different types [i.e., tellimagrandin II (monomer), hirtellin A (linear GOG-type dimer), hirtellin B (hellinoyl-type dimer), hirtellin C (macrocyclic-type dimer), and hirtellin T1 (linear GOG-type trimer)] in the resultant tissues in response to these factors were estimated by HPLC, and the optimal condition for production of these tannins were established. Shoots cultured on LS hormone-free medium promoted root development, and regenerated plants could adapt to ordinary soil and climate. Acclimatized and intact T. tetrandra plants that were collected in November and May, respectively, demonstrated seasonal differences in individual ellagitannin contents. HPLC comparison of individual ellagitannin contents in different plant materials (i.e., leaves, stems, and roots) of intact T. tetrandra plants is also reported. The results are discussed with respect to cellular deposition and biosynthetic relationship of tannins.
将柽柳的组织培养在添加了 30 g l(-1)蔗糖、2.13 mg l(-1)吲哚乙酸和 2.25 mg l(-1)苯甲腺嘌呤的林氏培养基上，可产生与柽柳科植物中完整植物中相同的鞣花单宁。这一点通过从培养组织的水相 Me2CO 提取物中分离出 14 种单体-四聚体鞣花单宁得到了证明。这是植物组织培养生产鞣花单宁四聚体的首次报道。考察了光照和某些培养基成分对组织生长和鞣花单宁生产的影响。通过 HPLC 估算了不同类型（即，tellimagrandin II（单体）、hirtellin A（线性 GOG 型二聚体）、hirtellin B（hellinoyl 型二聚体）、hirtellin C（大环型二聚体）和 hirtellin T1（线性 GOG 型三聚体））代表单宁的含量，建立了这些单宁的最佳生产条件。在 LS 无激素培养基上培养的枝条促进了根系的发育，再生的植物能够适应普通土壤和气候。分别于 11 月和 5 月采集的适应环境的完整柽柳植物在鞣花单宁含量上表现出季节性差异。还报告了不同完整柽柳植物材料（即叶片、茎和根）中鞣花单宁含量的 HPLC 比较。就单宁的细胞沉积和生物合成关系讨论了结果。
