Department of Microbiology, 32 Pearson Hall, Miami University, Oxford, OH 45056, USA.
J Gen Virol. 2011 Dec;92(Pt 12):2797-2802. doi: 10.1099/vir.0.035733-0. Epub 2011 Aug 10.
The recently discovered ascoviruses have a worldwide distribution. Here we report a new member of the family Ascoviridae, Spodoptera frugiperda ascovirus 1d (SfAV-1d) with a variable region in the genome. Restriction fragment length polymorphism, Southern hybridization and genome sequencing analyses confirmed that SfAV-1d and the earlier reported SfAV-1a are closely related but are not identical. The genome size of SfAV-1d is approximately 100 kbp, which is about 57 kbp smaller than SfAV-1a. The SfAV-1d genome has a major deletion of 14 kbp that corresponds to one of the inverted repeat (IR) regions of SfAV-1a. Cloning and sequencing revealed that the region flanking the deletion within the SfAV-1d genome is highly variable. In all the variants of this region, the whole IR region is missing, with 88.2 % of the variants missing part of or the whole adjacent SfAV-1a ORF71, 94.1 % missing part of or the whole of adjacent ORF72 and 64.6 % missing part of or the whole of ORF73.
最近发现的杆状病毒在世界范围内分布。在这里，我们报告了一种新的杆状病毒科成员，夜蛾科杆状病毒 1d（SfAV-1d），其基因组中有一个可变区。限制性片段长度多态性、Southern 杂交和基因组测序分析证实 SfAV-1d 和先前报道的 SfAV-1a 密切相关但并不相同。SfAV-1d 的基因组大小约为 100 kbp，比 SfAV-1a 小约 57 kbp。SfAV-1d 基因组有一个 14 kbp 的主要缺失，与 SfAV-1a 的一个反向重复（IR）区域相对应。克隆和测序显示，SfAV-1d 基因组中缺失区域的侧翼区域高度可变。在该区域的所有变体中，整个 IR 区域缺失，88.2%的变体缺失部分或整个相邻的 SfAV-1a ORF71，94.1%缺失部分或整个相邻的 ORF72，64.6%缺失部分或整个 ORF73。
