棉铃虫野田村病毒 3g 的基因组序列，该病毒分离自斜纹夜蛾。
Genomic sequence of Heliothis virescens ascovirus 3g isolated from Spodoptera exigua.
机构信息
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan, People's Republic of China.
出版信息
J Virol. 2012 Nov;86(22):12467-8. doi: 10.1128/JVI.02342-12.
Heliothis virescens ascovirus 3a (HvAV-3a), a member of the family Ascoviridae, has the highest diversity among ascovirus species that have been reported in Australia, Indonesia, China, and the United States. To understand the diversity and origin of this important ascovirus, the complete genome of the HvAV Indonesia strain (HvAV-3g), isolated from Spodoptera exigua, was determined to be 199,721 bp, with a G+C content of 45.9%. Therefore, HvAV-3g has the largest genome among the reported ascovirus genomes to date. There are 194 predicted open reading frames (ORFs) encoding proteins of 50 or more amino acid residues. In comparison to HvAV-3e reported from Australia, HvAV-3g has all the ORFs in HvAV-3e with 6 additional ORFs unique to HvAV-3g, including 1 peptidase C26 gene with the highest identity to Drosophila spp. and 2 gas vesicle protein U (GvpU) genes with identities to Bacillus megaterium. The five unique homologous regions (hrs) and 25 baculovirus repeat ORFs (bro) of HvAV-3g are highly variable.
棉铃虫野田村病毒 3a（HvAV-3a）是杆状病毒科的一员，在澳大利亚、印度尼西亚、中国和美国报道的所有杆状病毒种中具有最高的多样性。为了了解这种重要的杆状病毒的多样性和起源，我们对从斜纹夜蛾中分离得到的 HvAV 印度尼西亚株（HvAV-3g）的完整基因组进行了测定，其大小为 199721bp，G+C 含量为 45.9%。因此，HvAV-3g 是目前报道的杆状病毒基因组中最大的基因组。它有 194 个预测的开放阅读框（ORFs），编码 50 个或更多氨基酸残基的蛋白质。与从澳大利亚报道的 HvAV-3e 相比，HvAV-3g 拥有 HvAV-3e 中的所有 ORFs，还有 6 个 HvAV-3g 特有的 ORFs，包括 1 个与果蝇属亲缘关系最密切的肽酶 C26 基因和 2 个与巨大芽孢杆菌亲缘关系最密切的气腔蛋白 U（GvpU）基因。HvAV-3g 的 5 个独特同源区（hrs）和 25 个杆状病毒重复 ORFs（bro）高度可变。
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