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从甜菜夜蛾中分离出的烟青虫虹彩病毒 3h（HvAV-3h）的系统发育位置和复制动力学。

Phylogenetic position and replication kinetics of Heliothis virescens ascovirus 3h (HvAV-3h) isolated from Spodoptera exigua.

机构信息

Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan, China.

出版信息

PLoS One. 2012;7(7):e40225. doi: 10.1371/journal.pone.0040225. Epub 2012 Jul 5.

DOI:10.1371/journal.pone.0040225
PMID:22792245
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3390325/
Abstract

Insect-specific ascoviruses with a circular genome are distributed in the USA, France, Australia and Indonesia. Here, we report the first ascovirus isolation from Spodoptera exigua in Hunan, China. DNA-DNA hybridization to published ascoviruses demonstrated that the new China ascovirus isolate is a variant of Heliothis virescens ascovirus 3a (HvAV-3a), thus named HvAV-3h. We investigated the phylogenetic position, cell infection, vesicle production and viral DNA replication kinetics of HvAV-3h, as well as its host-ranges. The major capsid protein (MCP) gene and the delta DNA polymerase (DNA po1) gene of HvAV-3h were sequenced and compared with the available ascovirus isolates for phylogenetic analysis. This shows a close relationship with HvAV-3g, originally isolated from Indonesia, HvAV-3e from Australia and HvAV-3c from United States. HvAV-3h infection induced vesicle production in the SeE1 cells derived from S. exigua and Sf9 cells derived from S. frugiperda, resulting in more vesicles generated in Sf9 than SeE1. Viral DNA replication kinetics of HvAV-3h also demonstrated a difference between the two cell lines tested. HvAV-3h could readily infect three important insect pests Helicoverpa armigera (Hübner), Spodoptera exigua (Hübner) and Spodoptera litura (Fabricius) from two genera in different subfamilies with high mortalities.

摘要

具有圆形基因组的昆虫特异性虹彩病毒分布于美国、法国、澳大利亚和印度尼西亚。在此，我们报道了中国湖南首例分离自甜菜夜蛾的虹彩病毒。与已发表的虹彩病毒的 DNA-DNA 杂交表明，新的中国虹彩病毒分离株是棉铃虫虹彩病毒 3a（HvAV-3a）的变体，因此命名为 HvAV-3h。我们研究了 HvAV-3h 的系统发育位置、细胞感染、囊泡产生和病毒 DNA 复制动力学，以及它的宿主范围。我们对 HvAV-3h 的主要衣壳蛋白（MCP）基因和 delta DNA 聚合酶（DNA po1）基因进行了测序，并与可获得的虹彩病毒分离株进行了比较，用于系统发育分析。这表明它与 HvAV-3g（最初从印度尼西亚分离）、澳大利亚的 HvAV-3e 和美国的 HvAV-3c 密切相关。HvAV-3h 感染诱导 SeE1 细胞（来自甜菜夜蛾）和 Sf9 细胞（来自草地贪夜蛾）产生囊泡，导致 Sf9 细胞产生的囊泡多于 SeE1 细胞。HvAV-3h 的病毒 DNA 复制动力学也表明在两种测试细胞系之间存在差异。HvAV-3h 能够轻易感染来自两个不同亚科的两种重要害虫棉铃虫、甜菜夜蛾和斜纹夜蛾，死亡率很高。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e204/3390325/6f1a1e6d2486/pone.0040225.g008.jpg
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