新型烟青虫杆状病毒 3i （HvAV-3i）的基因组分析及囊膜蛋白重复开放阅读框（aros）的鉴定

Genomic analysis of a novel isolate Heliothis virescens ascovirus 3i (HvAV-3i) and identification of ascoviral repeat ORFs (aros).

机构信息

Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, 410128, Hunan, China.

Institute of Virology, College of Plant Protection, Hunan Agricultural University, Changsha, 410128, Hunan, China.

出版信息

Arch Virol. 2018 Oct;163(10):2849-2853. doi: 10.1007/s00705-018-3899-2. Epub 2018 Jun 14.

DOI:10.1007/s00705-018-3899-2

PMID:29948385

Abstract

Ascoviruses are circular double-stranded DNA viruses that infect insects. Herein we sequenced and analyzed the genome of the previously unrecorded ascovirus isolate Heliothis virescens ascovirus 3i (HvAV-3i). The genome size is 185,650 bp with 181 hypothetical open reading frames (ORFs). Additionally, definition based on ascovirus repeated ORFs (aros) is proposed; whereby the 29 aros from all sequenced Ascoviridae genomes are divided into six distinct groups. The topological relationship among the isolates of Heliothis virescens ascovirus 3a is (HvAV-3f, {HvAV-3h, [HvAV-3e, (HvAV-3g, HvAV-3i)]}) with every clade well supported by a Bayesian posterior probability of 1.00 and a Bootstrap value of 100%.

摘要

杆状病毒是感染昆虫的环状双链 DNA 病毒。在此，我们对先前未记录的斜纹夜蛾杆状病毒 3i（HvAV-3i）分离株进行了测序和基因组分析。基因组大小为 185650bp，包含 181 个假定的开放阅读框（ORF）。此外，还基于杆状病毒重复 ORF（aros）提出了定义；即所有已测序的杆状病毒科基因组中的 29 个 aros 分为六个不同的组。(HvAV-3f, {HvAV-3h, [HvAV-3e, (HvAV-3g, HvAV-3i)]})，每个分支都得到了贝叶斯后验概率为 1.00 和 100%的 Bootstrap 值的很好支持。