利用微悬臂梁传感器对淀粉样蛋白生长进行无标记检测。

Label-free detection of amyloid growth with microcantilever sensors.

作者信息

Knowles Tuomas P J, Shu Wenmiao, Huber François, Lang Hans Peter, Gerber Christoph, Dobson Christopher M, Welland Mark E

机构信息

Nanoscience Centre, University of Cambridge, J J Thomson Avenue, Cambridge CB3 0FF, UK.

出版信息

Nanotechnology. 2008 Sep 24;19(38):384007. doi: 10.1088/0957-4484/19/38/384007. Epub 2008 Aug 12.

DOI:10.1088/0957-4484/19/38/384007

PMID:21832567

Abstract

We present an approach for sensing protein aggregation using microcantilever systems. Results from both single cantilever experiments with internal reference and multicantilever array measurements with dedicated reference cantilevers are discussed. We show that in both cases protein aggregation on the sensor can be detected through associated changes in surface stress.

摘要

我们提出了一种使用微悬臂梁系统检测蛋白质聚集的方法。讨论了使用内部参考的单悬臂梁实验和使用专用参考悬臂梁的多悬臂梁阵列测量的结果。我们表明，在这两种情况下，都可以通过表面应力的相关变化检测传感器上的蛋白质聚集。