希腊高危人群中的血清阳性率：一项横断面研究。
Seroprevalence in a High-Risk Population in Greece: A Cross-Sectional Study.
作者信息
Andriopoulos Panagiotis, Floros Dimitrios, Gioti Natalia, Mariolis Anargiros, Rojas Gil Andrea Paola, Tsironi Maria
机构信息
Laboratory of Epidemiology and Prevention of Hemoglobinopathies, Acute and Chronic Diseases, Department of Nursing, University of Peloponnese, Valioti & Plateon, 23100 Sparta, Laconia, Greece.
Areopoli Health Center, Areopoli 23062, Laconia, Greece.
出版信息
Interdiscip Perspect Infect Dis. 2018 Dec 25;2018:8751921. doi: 10.1155/2018/8751921. eCollection 2018.
INTRODUCTION
Brucellosis is a zoonosis with high occupational risk. However, seroprevalence of antibodies among occupational groups is not known, since studies in endemic countries are rare.
METHODS
A cross-sectional seroprevalence study was conducted among livestock farmers in an endemic region in Greece. A low-risk group of individuals that just moved in the region was used as controls. Rose Bengal, Wright standard tube agglutination (STA) tests, and specific IgG and IgM antibodies using ELISA were evaluated; differences and odds ratios were calculated. Results were compared with studies from other endemic regions.
RESULTS
100 livestock farmers and family members and 34 first-year students were enrolled. Rose Bengal results were 18% positive versus 0% (p=0.007); Wright STAs for were 8% versus 2.9% (p=0.448) and for they were 2% versus 2.9% (p=0.588). ELISA IgG was positive in 8% of farmers versus 2.9% of students (p=0.448). Parallel testing with Rose Bengal and ELISA IgG was positive in 3% versus 0% (p=0.571). No significant odds ratios were calculated for Wright STAs and ELISA IgG.
CONCLUSIONS
Healthy livestock farmers may present with positive Rose Bengal test but this translates to true seroprevalence in only a small proportion. Livestock farmers have no significant seroprevalence that may obscure diagnosis of acute brucellosis in clinical settings.
引言
布鲁氏菌病是一种职业风险很高的人畜共患病。然而，由于在地方流行国家开展的研究较少，职业群体中抗体的血清流行率尚不清楚。
方法
在希腊一个地方流行地区对家畜养殖户开展了一项横断面血清流行率研究。将刚迁入该地区的低风险个体组用作对照。评估了玫瑰红试验、赖特标准试管凝集试验（STA）以及使用酶联免疫吸附测定法检测的特异性IgG和IgM抗体；计算了差异和比值比。将结果与其他地方流行地区的研究进行了比较。
结果
纳入了100名家畜养殖户及其家庭成员和34名一年级学生。玫瑰红试验结果的阳性率为18%，而对照组为0%（p = 0.007）；布鲁氏菌属的赖特STA阳性率为8%，对照组为2.9%（p = 0.448），流产布鲁氏菌的阳性率为2%，对照组为2.9%（p = 0.588）。酶联免疫吸附测定法检测的IgG在养殖户中的阳性率为8%，学生为2.9%（p = 0.448）。玫瑰红试验和酶联免疫吸附测定法检测的IgG并行检测的阳性率为3%，对照组为0%（p = 0.571）。未计算赖特STA和酶联免疫吸附测定法检测的IgG的显著比值比。
结论
健康的家畜养殖户可能玫瑰红试验呈阳性，但这仅在一小部分人中代表真正的血清流行率。家畜养殖户没有显著的血清流行率，这在临床环境中不会掩盖急性布鲁氏菌病的诊断。
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