Elner V M, Strieter R M, Elner S G, Baggiolini M, Lindley I, Kunkel S L
Department of Opthalmology, (Kellogg Eye Center), University of Michigan, Ann Arbor.
Am J Pathol. 1990 Apr;136(4):745-50.
The neural-derived retinal pigment epithelium (RPE) underlies the sensory retina and is central to both retinal homeostasis and many common retinal diseases. Retinal pigment epithelium cells are actively phagocytic and share several features with macrophages that have recently been shown to produce a neutrophil chemotactic factor (NCF), also known as interleukin-8, after cytokine stimulation. Because RPE cell responses to cytokines are largely unknown, human RPE cell NCF production was monitored after interleukin-1-beta (IL-1 beta), tumor necrosis factor-alpha, or lipopolysaccharide stimulation. RPE NCF mRNA expression and RPE production of biologically active NCF was time and concentration dependent. Maximal NCF mRNA expression occurred at 20 ng/ml for IL-1 beta. Messenger RNA expression in RPE cells and biologically active NCF in RPE cell supernatants were found 1 hour after stimulation and were maintained for 24 hours. These findings demonstrate that cytokine-stimulated RPE cells may evoke or augment neutrophil-mediated inflammation by synthesizing NCF, a cytokine that may be important in ocular disease mechanisms.
神经源性视网膜色素上皮（RPE）位于感觉视网膜之下，对视网膜内环境稳定和许多常见视网膜疾病都至关重要。视网膜色素上皮细胞具有活跃的吞噬作用，并且与巨噬细胞有一些共同特征，最近研究表明巨噬细胞在细胞因子刺激后会产生一种中性粒细胞趋化因子（NCF），也称为白细胞介素-8。由于RPE细胞对细胞因子的反应在很大程度上未知，因此在白细胞介素-1-β（IL-1β）、肿瘤坏死因子-α或脂多糖刺激后监测了人RPE细胞的NCF产生情况。RPE的NCF mRNA表达以及生物活性NCF的产生呈时间和浓度依赖性。IL-1β在20 ng/ml时出现最大NCF mRNA表达。刺激1小时后在RPE细胞中发现了mRNA表达，并且在RPE细胞上清液中发现了生物活性NCF，且持续24小时。这些发现表明，细胞因子刺激的RPE细胞可能通过合成NCF来引发或增强中性粒细胞介导的炎症，NCF这种细胞因子在眼部疾病机制中可能很重要。
