Pospischil A, Wood R L, Anderson T D
Physiopathology Research Unit, USDA, Ames, IA 50010.
Am J Vet Res. 1990 Apr;51(4):619-24.
Peroxidase-antiperoxidase immunoenzymatic labeling and immunogold labeling techniques were evaluated for microscopic detection and location of Salmonella organisms in tissues of experimentally infected swine. Salmonella typhimurium and Salmonella choleraesuis var kunzendorf were labeled specifically by the peroxidase-antiperoxidase technique in paraffin-embedded tissues of infected swine for conventional light microscopy and by postembedding immunogold labeling on ultrathin sections for electron microscopy. Salmonella typhimurium had a low tendency to invade the enteric mucosa and did not reveal any predilection for a specific intestinal location. Salmonella choleraesuis var kunzendorf, however, was located preferentially in colon and on the luminal surface of ileal M cells of Peyer patches and had a tendency to invade the enteric mucosa there.
采用过氧化物酶-抗过氧化物酶免疫酶标技术和免疫金标技术，对实验感染猪组织中的沙门氏菌进行显微镜检测和定位。在感染猪的石蜡包埋组织中，鼠伤寒沙门氏菌和猪霍乱沙门氏菌昆岑多夫变种通过过氧化物酶-抗过氧化物酶技术进行特异性标记，用于常规光学显微镜观察；在超薄切片上通过包埋后免疫金标技术进行标记，用于电子显微镜观察。鼠伤寒沙门氏菌侵袭肠道黏膜的倾向较低，且未显示出对特定肠道位置的偏好。然而，猪霍乱沙门氏菌昆岑多夫变种优先定位于结肠以及派伊尔结回肠M细胞的腔面，并倾向于侵袭该处的肠道黏膜。
