Department of Clinical Laboratory Medicine, School of Medicine, Juntendo University, Tokyo, Japan.
Mycopathologia. 2012 Jan;173(1):21-5. doi: 10.1007/s11046-011-9458-y. Epub 2011 Aug 12.
We evaluated antifungal activity as assessed by the contact time in topical use of gentian violet (GV) and povidone-iodine (PI) against Candida strains. A total of 102 yeast isolates were used in this study. A markedly lower minimal inhibitory concentration (MIC)(90) of GV than of PI was detected for all yeast isolates. No remarkable difference in the MICs was observed among the identical strains isolated from different clinical sites for both GV and PI. Although the minimal fungicidal activities (MFCs) of PI were identical for all tested time points, the fungicidal activity of GV decreased during the time course of incubation. These results indicate that, whereas GV is more effective than PI, the topical disinfectant efficacy of GV should be estimated using the MFC(5 min) and not the MIC or the MFC(24 h) for overall prevention of catheter-related bloodstream infections and oral infections.
我们评估了接触时间对局部使用龙胆紫（GV）和聚维酮碘（PI）抗念珠菌的抗真菌活性的影响。本研究共使用了 102 株酵母分离株。与 PI 相比，所有酵母分离株的 GV 的最小抑菌浓度（MIC）（90）明显更低。对于 GV 和 PI，从不同临床部位分离的相同菌株的 MIC 之间没有明显差异。虽然 PI 的最小杀菌浓度（MFC）在所有测试时间点均相同，但 GV 的杀菌活性在孵育过程中随时间下降。这些结果表明，虽然 GV 比 PI 更有效，但应使用 MFC（5 分钟）而不是 MIC 或 MFC（24 小时）来评估 GV 的局部消毒效果，以全面预防导管相关血流感染和口腔感染。
