Nagayama Ariaki
Department of Microbiology and Immunology, School of Medicine, Fukuoka University, 7-45-1 Nanakuma, Fukuoka 814-0180, Japan.
J Infect Chemother. 2006 Apr;12(2):73-9. doi: 10.1007/s10156-005-0426-7.
Recently we have heard warnings of an outbreak of a highly pathogenic avian influenza virus (H5N1). Although, to prevent such infections we must prepare anti-viral drugs and type-specific vaccines against influenza, we need various simple and effective protection methods, such as the use of face masks for public health. Also, in any consideration of bacterial infections, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococci (VRE), and multidrug-resistant Pseudomonas aeruginosa (MDRP) also pose serious concerns which must be addressed. I examined the antiviral activity of gentian violet (GV) and GV-dyed cloth against the influenza A (H1N1) virus. Time-kill studies were carried out, and the virus titer was determined based on the 50% tissue culture infective dose (TCID50). The minimum inhibitory concentrations (MICs) of GV against bacteria were also determined, and the killing activities of the GV-dyed cloth were judged from viable cell counts. GV immediately killed the influenza A virus and this was confirmed by electron microscopy. Moreover, cloth dyed with a combination of GV and copper showed not only excellent antiviral activity but also prominent bactericidal activities.
最近，我们听闻了高致病性禽流感病毒（H5N1）爆发的警告。尽管为预防此类感染，我们必须准备抗病毒药物和针对流感的特定类型疫苗，但我们也需要各种简单有效的防护方法，比如使用口罩以保障公众健康。此外，在任何关于细菌感染的考量中，耐甲氧西林金黄色葡萄球菌（MRSA）、耐万古霉素肠球菌（VRE）以及多重耐药铜绿假单胞菌（MDRP）也引发了必须加以应对的严重问题。我研究了龙胆紫（GV）以及用GV染色的布料对甲型流感病毒（H1N1）的抗病毒活性。进行了时间杀灭研究，并基于50%组织培养感染剂量（TCID50）测定病毒滴度。还测定了GV对细菌的最低抑菌浓度（MIC），并通过活菌计数判断用GV染色的布料的杀菌活性。GV能立即杀灭甲型流感病毒，这一点通过电子显微镜得到了证实。此外，用GV和铜的组合染色的布料不仅显示出优异的抗病毒活性，还具有显著的杀菌活性。
