Liu Tiezheng, Li Xi, Jiang Qingmei, Zhu Huiwen, Wang Guichun
Marine College, Shandong University at Weihai, Weihai 264200, China.
Zhongguo Zhong Yao Za Zhi. 2011 May;36(10):1358-61.
The synergic and decreasing toxic effects of mineral water and Chinese herbal compound preparation (MWCHCP) on cisplatin were investigated in sarcoma 180 (S180) mice.
The S180 mice were treated for 5 days with intraperitoneal injection of cisplatin(7.33 mg x kg(-1)) and oral administration of MWCHCP(1 925, 3 850, 7 700 mg x kg(-1)). Then the mice were killed and the tumor growth inhibition rate, organ index, diarrhea index were determined. Observe pathological sections of stomach to study the protective effect of MWCHCP. Reverse transcription-polymerase chain reaction (RT-PCR) was applied to investigate the tumour necrosis factor-alpha (TNF-alpha) expression level of the intestine.
Combining with cisplatin and MWCHCP caused a tendency of increasing the tumor growth inhibition rate and significant attenution of cisplatin-induced diarrhea, visceral organ injury, gastric mucosal injury and decreased TNF-alpha mRNA level of intestine.
The present findings suggest that MWCHCP increases the inhibition rate of tumor growth of cisplatin and has a beneficial influence on gastrointestinal lesion induced by cisplatin.
在肉瘤180（S180）小鼠中研究矿泉水与中药复方制剂（MWCHCP）对顺铂的增效及减毒作用。
S180小鼠腹腔注射顺铂（7.33 mg·kg⁻¹）并口服MWCHCP（1925、3850、7700 mg·kg⁻¹），连续处理5天。然后处死小鼠，测定肿瘤生长抑制率、脏器指数、腹泻指数。观察胃组织病理切片以研究MWCHCP的保护作用。应用逆转录聚合酶链反应（RT-PCR）检测肠道肿瘤坏死因子-α（TNF-α）的表达水平。
顺铂与MWCHCP联合使用有提高肿瘤生长抑制率的趋势，且能显著减轻顺铂所致的腹泻、内脏器官损伤、胃黏膜损伤，并降低肠道TNF-α mRNA水平。
本研究结果表明，MWCHCP可提高顺铂对肿瘤生长的抑制率，并对顺铂所致胃肠道损伤有有益影响。
