Chongqing University, College of Bioengineering, Department of Bioengineering, Key Laboratory of Biorheological Science and Technology, Ministry of Education, Chongqing 400030, China.
Physiol Plant. 2011 Nov;143(3):235-45. doi: 10.1111/j.1399-3054.2011.01502.x. Epub 2011 Sep 20.
Citrus Huanglongbing (HLB) has been threatening citrus production worldwide. In this study, a comparative proteomic approach was applied to understand the pathogenic process of HLB in affected sweet orange leaves. Using the isobaric tags for relative and absolute quantification (iTRAQ) technique, we identified 686 unique proteins in the mature leaves of both mock-inoculated and diseased 'Madam Vinous' sweet orange plants. Of the identified proteins, 20 and 10 were differentially expressed in leaves with and without symptoms of HLB (fold change > 2.5), respectively, compared with mock-inoculated controls. Most significantly, upregulated proteins were involved in stress/defense response, such as four miraculin-like proteins, chitinase, Cu/Zn superoxide dismutase and lipoxygenase. Microarray analysis also showed that stress-related genes were significantly upregulated at the transcriptional level. For example, remarkable upregulations of miraculin-like proteins and Cu/Zn superoxide dismutase transcripts were observed. Moreover, the transcriptional patterns of miraculin-like protein 1 and Cu/Zn superoxide dismutase were examined at different stages of HLB disease development. Combined with the transcriptomic data, the proteomic data can provide an enhanced understanding of citrus stress/defense responses to HLB.
柑橘黄龙病（HLB）一直威胁着世界柑橘产业。在这项研究中，采用比较蛋白质组学方法来了解感病甜橙叶片中 HLB 的致病过程。使用同位素相对标记与绝对定量（iTRAQ）技术，我们鉴定了模拟接种和感病‘Madam Vinous’甜橙植株成熟叶片中的 686 种独特蛋白质。与模拟接种对照相比，在有和没有 HLB 症状的叶片中，分别有 20 和 10 种蛋白质差异表达（变化倍数>2.5）。最显著的是，上调的蛋白质参与应激/防御反应，如 4 种神秘果素样蛋白、几丁质酶、Cu/Zn 超氧化物歧化酶和脂氧合酶。微阵列分析还表明，在转录水平上应激相关基因显著上调。例如，神秘果素样蛋白和 Cu/Zn 超氧化物歧化酶的转录本显著上调。此外，还检测了神秘果素样蛋白 1 和 Cu/Zn 超氧化物歧化酶在 HLB 发病不同阶段的转录模式。结合转录组数据，蛋白质组数据可以提供对柑橘对 HLB 应激/防御反应的更深入理解。
