Department of Physiology, China Pharmaceutical University, 24 Tongjiaxiang Street, Nanjing 210009, PR China.
Eur J Pharmacol. 2011 Nov 16;670(1):148-53. doi: 10.1016/j.ejphar.2011.07.037. Epub 2011 Aug 5.
Maslinic acid is a triterpenoid compound present in plants of Olea europaea. This compound has been reported to have potent antioxidant, anti-cancer, anti-HIV and anti-inflammatory activities. In this study, we investigated the neuroprotective effect of maslinic acid and its mechanism of action. With presence or absence of maslinic acid, cortical neurons were subjected to 1h of oxygen-glucose deprivation and 24h of reoxygenation. Cell injury was determined by lactate dehydrogenase (LDH) measurement and 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. Neuronal apoptosis was evaluated by flow cytometry assay, caspase-3 expression/activity, caspase-9 activity and Bcl-2/Bax ratio. Nitric Oxide (NO) production and inducible nitric oxide synthase (iNOS) expression were also detected. Results showed that maslinic acid dose-dependently ameliorated neuron injury and apoptosis. Maslinic acid treatment normalized the caspase expression/activation and increased the Bcl-2/Bax ratio. In addition, maslinic acid inhibited oxygen-glucose deprivation-induced NO production and iNOS expression. These results indicated that maslinic acid has beneficial effects on hypoxic neurons by suppressing iNOS activation, which may, in turn, provide neuroprotection.
齐墩果酸是一种存在于油橄榄植物中的三萜类化合物。该化合物已被报道具有强大的抗氧化、抗癌、抗 HIV 和抗炎活性。在这项研究中，我们研究了齐墩果酸的神经保护作用及其作用机制。在有或没有齐墩果酸的情况下，皮质神经元接受 1 小时的氧葡萄糖剥夺和 24 小时的复氧。通过乳酸脱氢酶（LDH）测量和 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）测定来确定细胞损伤。通过流式细胞术测定、半胱天冬酶-3 表达/活性、半胱天冬酶-9 活性和 Bcl-2/Bax 比值评估神经元凋亡。还检测了一氧化氮（NO）产生和诱导型一氧化氮合酶（iNOS）表达。结果表明，齐墩果酸可剂量依赖性地改善神经元损伤和凋亡。齐墩果酸处理使半胱天冬酶的表达/激活正常化，并增加了 Bcl-2/Bax 比值。此外，齐墩果酸抑制了氧葡萄糖剥夺诱导的 NO 产生和 iNOS 表达。这些结果表明，齐墩果酸通过抑制 iNOS 激活对缺氧神经元具有有益作用，从而提供神经保护。
